基因组技术在动物抗生素耐药性的检测和监测中的应用。
Application of genomic technologies to measure and monitor antibiotic resistance in animals.
机构信息
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China.
State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
出版信息
Ann N Y Acad Sci. 2017 Jan;1388(1):121-135. doi: 10.1111/nyas.13296. Epub 2016 Dec 20.
One of the richest reservoirs of antibiotic-resistant bacteria and genes, animal intestinal microbiota contributes to the spread of antibiotic resistance in the environment and, potentially, to human pathogens. Both culture-based genomic technology and culture-independent metagenomics have been developed to investigate the abundance and diversity of antibiotic resistance genes. The characteristics, strengths, limitations, and challenges of these genomic approaches are discussed in this review in the context of antibiotic resistance in animals. We also discuss the advances in single-cell genomics and its potential for surveillance of antibiotic resistance in animals.
动物肠道微生物群是抗生素耐药菌和基因的最丰富资源之一，它有助于抗生素耐药性在环境中的传播，并可能导致人类病原体的出现。基于培养的基因组技术和非培养的宏基因组学已经被开发出来，用于研究抗生素耐药基因的丰度和多样性。本文讨论了这些基因组方法的特点、优势、局限性和挑战，以及它们在动物抗生素耐药性方面的应用。我们还讨论了单细胞基因组学的进展及其在动物抗生素耐药性监测方面的潜力。
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