Wang Liling, Dai Yang, Chen Wanping, Shao Yanchun, Chen Fusheng
College of Food Science and Technology, Huazhong Agricultural University , Wuhan, 430070, Hubei Province, PR China.
College of Life Science, Production & Construction Group Key Laboratory of Special Agricultural Products Further Processing in Southern Xinjiang, Tarim University , Alar 843300, Xinjiang, China.
J Agric Food Chem. 2016 Dec 21;64(50):9506-9514. doi: 10.1021/acs.jafc.6b04056. Epub 2016 Dec 8.
Light is a crucial environmental signal for fungi. In this work, the effects of different light intensities and colors on biomass, Monascus pigments (MPs) and citrinin production of Monascus ruber M7 were investigated. We have demonstrated that low intensity of blue light (500 lx) decreased Monascus biomass, increased MPs accumulation via upregulation of MpigA, MpigB, and MpigJ genes expression, but had no significant influence on citrinin production. High intensity of blue light (1500 lx) decreased citrinin accumulation but had no significant influence on biomass and MPs production after 14 days cultivation. Low intensity of green light (500 lx) stimulated citrinin production via upregulation of pksCT, mrl1, mrl2, and ctnA genes expression. One putative red light photoreceptor and two putative green light photoreceptors were identified in M. ruber M7. These observations will not only guide the practical production of Monascus but also contribute to our understanding light effects on Monascus.
光对真菌而言是一种至关重要的环境信号。在本研究中，我们探究了不同光强和颜色对红曲霉菌M7的生物量、红曲色素（MPs）及桔霉素产生的影响。我们发现，低强度蓝光（500勒克斯）会降低红曲霉生物量，通过上调MpigA、MpigB和MpigJ基因的表达增加MPs积累，但对桔霉素产生没有显著影响。高强度蓝光（1500勒克斯）会降低桔霉素积累，但在培养14天后对生物量和MPs产生没有显著影响。低强度绿光（500勒克斯）通过上调pksCT、mrl1、mrl2和ctnA基因的表达刺激桔霉素产生。在红曲霉菌M7中鉴定出一个假定的红光光感受器和两个假定的绿光光感受器。这些发现不仅将指导红曲霉菌的实际生产，也有助于我们理解光对红曲霉菌的影响。
