State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China.
State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China.
Sci Total Environ. 2017 Feb 15;580:1175-1184. doi: 10.1016/j.scitotenv.2016.12.075. Epub 2016 Dec 18.
Antibiotics are widely used in mariculture industry, and this study attempts to determine the extent of water and sediment pollution by antibiotic residues in 13 major mariculture sites in China. Through chemical and molecular biology analysis, the results showed that the total concentrations of sulfonamides and tetracyclines were in the range 62.0-373.8ngL and 0.2-259.1ngL respectively in water samples, and in the range 0.19-1.59ngg dry weight and 3.45-74.84ngg dry weight respectively, in sediments samples. The occurrence of antibiotic resistance genes (ARGs) was detected in all sites. Compared with the tetracyclines resistance genes, the absolute copy number and relative abundance of the sulfonamides resistance genes were 4.3 times and 2.3 times higher in water and sediment from the mariculture sites, with the dominant resistance genes being sul2. The abundance of sul3 in the water phase was significantly correlated with the concentrations of sulfamerazine, while the abundance of sul2 in the sediment phase was significantly with sulfadiazine concentrations. The abundance of tetM in the sediment phase was significantly correlated with the concentrations of oxytetracycline. The findings demonstrate the persistence of antibiotic residues and ARGs in major mariculture sites in Southeast China.
抗生素在水产养殖业中被广泛使用，本研究试图确定中国 13 个主要水产养殖区的抗生素残留对水和沉积物污染的程度。通过化学和分子生物学分析，结果表明，水样中磺胺类和四环素的总浓度分别在 62.0-373.8ng/L 和 0.2-259.1ng/L 范围内，而沉积物样本中磺胺类和四环素的浓度分别在 0.19-1.59ng/g 干重和 3.45-74.84ng/g 干重范围内。在所有地点都检测到了抗生素耐药基因（ARGs）的存在。与四环素耐药基因相比，磺胺类耐药基因在水产养殖区水相和底泥中的绝对拷贝数和相对丰度分别高 4.3 倍和 2.3 倍，优势耐药基因是 sul2。磺胺甲恶唑浓度与水相中 sul3 的丰度显著相关，磺胺嘧啶浓度与底泥相中 sul2 的丰度显著相关。土霉素浓度与底泥相中 tetM 的丰度显著相关。研究结果表明，抗生素残留和 ARGs 在华东地区主要水产养殖区具有持久性。
