The City College of the City University of New York, Institute of Ultrafast Spectroscopy and Lasers, Departments of Physics and Electrical Engineering, 160 Convent Avenue, Room MR 201, New York, New York 10031-9101, United States.
The City University of New York, Borough of Manhattan Community College, 199 Chambers Street, N682, New York, New York 10007-1097, United States.
J Biomed Opt. 2016 Dec 1;21(12):127006. doi: 10.1117/1.JBO.21.12.127006.
A clear correlation has been observed between the resonance Raman (RR) spectra of plaques in the aortic tunica intimal wall of a human corpse and three states of plaque evolution: fibrolipid plaques, calcified and ossified plaques, and vulnerable atherosclerotic plaques (VPs). These three states of atherosclerotic plaque lesions demonstrated unique RR molecular fingerprints from key molecules, rendering their spectra unique with respect to one another. The vibrational modes of lipids, cholesterol, carotenoids, tryptophan and heme proteins, the amide I, II, III bands, and methyl/methylene groups from the intrinsic atherosclerotic VPs in tissues were studied. The salient outcome of the investigation was demonstrating the correlation between RR measurements of VPs and the thickness measurements of fibrous caps on VPs using standard histopathology methods, an important metric in evaluating the stability of a VP. The RR results show that VPs undergo a structural change when their caps thin to 66 ?? ? m , very close to the 65 - ? m empirical medical definition of a thin cap fibroatheroma plaque, the most unstable type of VP.
已经观察到人类尸体主动脉内膜中层斑块的共振拉曼（RR）光谱与斑块演化的三种状态之间存在明显的相关性：纤维脂斑、钙化和骨化斑块以及易损动脉粥样硬化斑块（VPs）。这三种动脉粥样硬化斑块病变表现出关键分子的独特 RR 分子指纹，使得它们的光谱彼此独特。研究了组织中脂质、胆固醇、类胡萝卜素、色氨酸和血红素蛋白的振动模式、酰胺 I、II、III 带以及内在动脉粥样硬化 VP 中的甲基/亚甲基基团。调查的突出结果表明，使用标准组织病理学方法，RR 测量的 VP 与 VP 上纤维帽的厚度测量之间存在相关性，这是评估 VP 稳定性的重要指标。RR 结果表明，当它们的帽层变薄到 66μm 时，VP 会发生结构变化，非常接近 65μm 的薄帽纤维帽脂瘤的经验医学定义，这是最不稳定的 VP 类型。
