Acharya Anirudh B, Thakur Srinath, Muddapur M V, Kulkarni Raghavendra D
Department of Periodontics, S.D.M. College of Dental Sciences & Hospital, Dharwad-580009, Karnataka, India.
Department of Microbiology, S.D.M. College of Medical Sciences & Hospital, Dharwad-580009, Karnataka, India.
Curr Diabetes Rev. 2018;14(2):182-188. doi: 10.2174/1573399812666161220144011.
Cytokine dysregulation plays an important role in Type 2 Diabetes Mellitus (T2DM) and Chronic Periodontitis (CP) with a commonality in pathogenic mechanisms. CP is considered the sixth complication of diabetes and may have an increased influence on systemic levels of cytokines in individuals with T2DM.
This study investigated two pro-, and two presumed anti-inflammatory cytokines and their ratios in the serum of healthy individuals, in chronic periodontitis with and without T2DM with, and without CP and in CP alone aimed at evaluating the systemic inflammatory burden of a local oral infection.
Eighty participants were divided equally into four groups as healthy volunteers (H) and patients having T2DM with, and without CP (T2DM+CP, and T2DM) and only CP (CP). Serum samples were collected to measure glycated hemoglobin (HbA1c), Random Blood Sugar (RBS) and also Tumor Necrosis Factor (TNF)-α, Interleukin (IL)-4, -6 - 10 were assessed using commercially available ELISA kits.
The cytokines were detected in all groups. Significant differences were observed between groups for all the clinical, biochemical parameters and cytokines. Cytokine levels and the ratios showed significant correlations. The ratios of the cytokines differed significantly amongst groups, were highest in T2DM+CP.
In this study, the cytokine ratios provided a qualitative profile along with the absolute levels in T2DM with periodontitis, indicative of an intensified systemic inflammatory state.
细胞因子失调在2型糖尿病（T2DM）和慢性牙周炎（CP）中起重要作用，二者在致病机制上具有共性。CP被认为是糖尿病的第六大并发症，可能对T2DM患者的细胞因子全身水平产生更大影响。
本研究调查了两种促炎细胞因子和两种假定的抗炎细胞因子及其在健康个体血清中的比例，这些个体患有或未患有T2DM、患有或未患有CP以及仅患有CP，旨在评估局部口腔感染的全身炎症负担。
80名参与者被平均分为四组，分别为健康志愿者（H）、患有T2DM且患有或未患有CP的患者（T2DM+CP和T2DM）以及仅患有CP的患者（CP）。采集血清样本以测量糖化血红蛋白（HbA1c）、随机血糖（RBS），并使用市售ELISA试剂盒评估肿瘤坏死因子（TNF）-α、白细胞介素（IL）-4、-6、-10。
所有组均检测到细胞因子。在所有临床、生化参数和细胞因子方面，各组之间均观察到显著差异。细胞因子水平与比例显示出显著相关性。细胞因子比例在各组之间存在显著差异，在T2DM+CP组中最高。
在本研究中，细胞因子比例与T2DM合并牙周炎时的绝对水平一起提供了一个定性概况，表明全身炎症状态加剧。
