Ferro Camila G, Zerbini F Murilo, Navas-Castillo Jesús, Fiallo-Olivé Elvira
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Consejo Superior de Investigaciones Científicas-Universidad de Málaga (IHSM-CSIC-UMA), 29750 Algarrobo-Costa, Málaga, Spain.
Departmento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa 36570-900, MG, Brazil.
Microorganisms. 2021 May 10;9(5):1018. doi: 10.3390/microorganisms9051018.
Sweepoviruses are begomoviruses (genus , family ) with ssDNA genomes infecting sweet potato and other species of the family Convolvulaceae. Deltasatellites (genus , family ) are small-size non-coding DNA satellites associated with begomoviruses. In this study, the genetic diversity of deltasatellites associated with sweepoviruses infecting plants was analyzed by further sampling the populations where the deltasatellite sweet potato leaf curl deltasatellite 1 (SPLCD1) was initially found, expanding the search to other geographical areas in southern continental Spain and the Canary Islands. The sweepoviruses present in the samples coinfected with deltasatellites were also fully characterized by sequencing in order to define the range of viruses that could act as helper viruses in nature. Additionally, experiments were performed to assess the ability of a number of geminivirids (the monopartite tomato leaf deformation virus and the bipartite NW begomovirus Sida golden yellow vein virus, the bipartite OW begomovirus tomato leaf curl New Delhi virus, and the curtovirus beet curly top virus) to transreplicate SPLCD1 in their natural plant hosts or the experimental host . The results show that SPLCD1 can be transreplicated by all the geminivirids assayed in and by tomato leaf curl New Delhi virus in zucchini. The presence of SPLCD1 did not affect the symptomatology caused by the helper viruses, and its effect on viral DNA accumulation depended on the helper virus-host plant combination.
帚状病毒是双生病毒科（属，科）的单链DNA病毒，可感染甘薯和旋花科的其他物种。δ卫星（属，科）是与双生病毒相关的小尺寸非编码DNA卫星。在本研究中，通过进一步对最初发现δ卫星甘薯卷叶δ卫星1（SPLCD1）的种群进行采样，并将搜索范围扩大到西班牙大陆南部和加那利群岛的其他地理区域，分析了与感染植物的帚状病毒相关的δ卫星的遗传多样性。还通过测序对与δ卫星共感染的样本中存在的帚状病毒进行了全面表征，以确定在自然界中可作为辅助病毒的病毒范围。此外，还进行了实验，以评估一些双生病毒（单分体番茄叶变形病毒和双分体西北双生病毒 sida 金黄脉病毒、双分体旧世界双生病毒番茄卷叶新德里病毒以及曲顶病毒甜菜曲顶病毒）在其自然植物宿主或实验宿主中反式复制SPLCD1的能力。结果表明，在本研究中所有检测的双生病毒以及番茄卷叶新德里病毒在西葫芦中均可反式复制SPLCD1。SPLCD1的存在不影响辅助病毒引起的症状，其对病毒DNA积累的影响取决于辅助病毒-宿主植物组合。
