Yu Jia-Lu, Song Qi-Fang, Xie Zhi-Wei, Jiang Wen-Hui, Chen Jia-Hui, Fan Hui-Feng, Xie Ya-Ping, Lu Gen
Guangzhou Women and Children's Medical Center, Guangzhou Medical University.
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University.
Jpn J Infect Dis. 2017 Sep 25;70(5):571-578. doi: 10.7883/yoken.JJID.2016.355. Epub 2016 Dec 22.
Mycoplasma pneumoniae (MP) is a leading cause of community-acquired pneumonia in children and young adults. Although MP pneumonia is usually benign and self-limited, in some cases it can develop into life-threating refractory MP pneumonia (RMPP). However, the pathogenesis of RMPP is poorly understood. The identification and characterization of proteins related to RMPP could provide a proof of principle to facilitate appropriate diagnostic and therapeutic strategies for treating paients with MP. In this study, we used a quantitative proteomic technique (iTRAQ) to analyze MP-related proteins in serum samples from 5 patients with RMPP, 5 patients with non-refractory MP pneumonia (NRMPP), and 5 healthy children. Functional classification, sub-cellular localization, and protein interaction network analysis were carried out based on protein annotation through evolutionary relationship (PANTHER) and Cytoscape analysis. A total of 260 differentially expressed proteins were identified in the RMPP and NRMPP groups. Compared to the control group, the NRMPP and RMPP groups showed 134 (70 up-regulated and 64 down-regulated) and 126 (63 up-regulated and 63 down-regulated) differentially expressed proteins, respectively. The complex functional classification and protein interaction network of the identified proteins reflected the complex pathogenesis of RMPP. Our study provides the first comprehensive proteome map of RMPP-related proteins from MP pneumonia. These profiles may be useful as part of a diagnostic panel, and the identified proteins provide new insights into the pathological mechanisms underlying RMPP.
肺炎支原体（MP）是儿童和青年社区获得性肺炎的主要病因。尽管MP肺炎通常为良性且具有自限性，但在某些情况下可发展为危及生命的难治性MP肺炎（RMPP）。然而，RMPP的发病机制尚不清楚。鉴定和表征与RMPP相关的蛋白质可为制定针对MP肺炎患者的适当诊断和治疗策略提供原理证明。在本研究中，我们使用定量蛋白质组学技术（iTRAQ）分析了5例RMPP患者、5例非难治性MP肺炎（NRMPP）患者和5名健康儿童血清样本中的MP相关蛋白质。基于通过进化关系进行蛋白质注释（PANTHER）和Cytoscape分析，进行了功能分类、亚细胞定位和蛋白质相互作用网络分析。在RMPP组和NRMPP组中总共鉴定出260种差异表达蛋白质。与对照组相比，NRMPP组和RMPP组分别显示出134种（70种上调和64种下调）和126种（63种上调和63种下调）差异表达蛋白质。所鉴定蛋白质的复杂功能分类和蛋白质相互作用网络反映了RMPP的复杂发病机制。我们的研究提供了首个来自MP肺炎的RMPP相关蛋白质的综合蛋白质组图谱。这些图谱可能作为诊断指标的一部分有用，并且所鉴定的蛋白质为RMPP潜在的病理机制提供了新见解。
