Zhang Xiaoqian, Li Kecheng, Liu Song, Zou Ping, Xing Ronge, Yu Huahua, Chen Xiaolin, Qin Yukun, Li Pengcheng
Key Laborotory Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences , Qingdao 266071, China.
University of Chinese Academy of Sciences , Beijing 100049, China.
J Agric Food Chem. 2017 Jan 18;65(2):501-509. doi: 10.1021/acs.jafc.6b03665. Epub 2017 Jan 3.
Seven chitooligomers (COSs) with determined degrees of polymerization (DPs) (chitotetraose to chitooctaose, DP 8-10, DP 10-12) and a heterogeneous COS with various DPs were first applied to explore the relationship between the DP of COSs and their effect on the growth of wheat seedlings under salt stress. The results showed that COS could promote the growth of wheat seedlings under salt stress. Moreover, chitohexaose, chitoheptaose, and chitooctaose exhibited stronger activity compared with other COS samples, which suggested that their activity had a close relationship with the DP. After 10 days of treatment with chitohexaose, chitoheptaose, and chitooctaose, the photosynthetic parameters were obviously improved. The soluble sugar and proline contents were improved by 26.7-53.3 and 43.6-70.2%, respectively, whereas the concentration of malondialdehyde (MDA) was reduced by 36.8-49.6%. In addition, the antioxidant enzyme activities were clearly activated. At the molecular level, the results revealed that they could obviously induce the expression of Na/H antiporter genes.
首先应用七种具有确定聚合度（DPs）的壳寡糖（COSs）（壳四糖至壳八糖、DP 8 - 10、DP 10 - 12）以及一种具有不同DPs的异质COS，以探究COSs的DP与其在盐胁迫下对小麦幼苗生长影响之间的关系。结果表明，COS能够促进盐胁迫下小麦幼苗的生长。此外，壳六糖、壳七糖和壳八糖相较于其他COS样品表现出更强的活性，这表明它们的活性与DP密切相关。用壳六糖、壳七糖和壳八糖处理10天后，光合参数明显改善。可溶性糖和脯氨酸含量分别提高了26.7 - 53.3%和43.6 - 70.2%，而丙二醛（MDA）浓度降低了36.8 - 49.6%。此外，抗氧化酶活性明显被激活。在分子水平上，结果显示它们能够明显诱导Na/H反向转运蛋白基因的表达。
