Marine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Marine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Carbohydr Polym. 2017 Mar 15;160:194-202. doi: 10.1016/j.carbpol.2016.12.058. Epub 2016 Dec 23.
In this study, six fully deacetylated single chitooligomers (COSs, chitobiose to chitoheptaose) and one fully deacetylated COS mixture were applied to wheat seedlings to investigate their effects on the plants' defence response under chilling stress. The results showed that exogenous COS with different degrees of polymerization (DPs) could promote the growth of plants, decrease the concentration of malondialdehyde (MDA), increase the content of chlorophyll and modulate the activities of antioxidant enzymes. The analysis of genes expression suggested that COS could alleviate the damage of chilling stress by effectively regulating the expression of antioxidant enzyme genes. Furthermore, chitohexaose and chitoheptaose had the most effective activities of alleviating chilling stress to wheat seedlings, which suggested the DP played important roles in the activities of COS. The close relationship between the activities of COSs and their DPs reveals that the activities of COSs are dependent upon a specific structure to interact with elicitor receptors on plant cell membrane.
在这项研究中，六种完全脱乙酰的单壳寡糖（COS，从二糖到七糖）和一种完全脱乙酰的 COS 混合物被应用于小麦幼苗，以研究它们在冷胁迫下对植物防御反应的影响。结果表明，具有不同聚合度（DP）的外源 COS 可以促进植物的生长，降低丙二醛（MDA）的浓度，增加叶绿素的含量，并调节抗氧化酶的活性。基因表达分析表明，COS 通过有效调节抗氧化酶基因的表达，可以缓解冷胁迫对植物的损伤。此外，壳六糖和壳七糖对小麦幼苗缓解冷胁迫的效果最为显著，这表明 DP 在 COS 的活性中起着重要作用。COS 活性与其 DP 之间的密切关系表明，COS 的活性取决于与植物细胞膜上的受体相互作用的特定结构。
