Atmanli Ayhan, Domian Ibrahim John
Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA; Department of Biomedical Engineering, Tufts University, Medford, MA, USA.
Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USA; Harvard Stem Cell Institute, Cambridge, MA, USA.
Trends Cell Biol. 2017 May;27(5):352-364. doi: 10.1016/j.tcb.2016.11.010. Epub 2016 Dec 19.
The advent of human pluripotent stem cell (hPSC) biology has opened unprecedented opportunities for the use of tissue engineering to generate human cardiac tissue for in vitro study. Engineering cardiac constructs that recapitulate human development and disease requires faithful recreation of the cardiac niche in vitro. Here we discuss recent progress in translating the in vivo cardiac microenvironment into PSC models of the human heart. We review three key physiologic features required to recreate the cardiac niche and facilitate normal cardiac differentiation and maturation: the biochemical, biophysical, and bioelectrical signaling cues. Finally, we discuss key barriers that must be overcome to fulfill the promise of stem cell biology in preclinical applications and ultimately in clinical practice.
人类多能干细胞(hPSC)生物学的出现为利用组织工程技术生成用于体外研究的人类心脏组织带来了前所未有的机遇。构建能够重现人类发育和疾病过程的心脏构建体需要在体外忠实地重现心脏微环境。在此,我们讨论了将体内心脏微环境转化为人类心脏PSC模型的最新进展。我们回顾了重现心脏微环境并促进正常心脏分化和成熟所需的三个关键生理特征:生化、生物物理和生物电信号线索。最后,我们讨论了在临床前应用乃至最终临床实践中实现干细胞生物学前景必须克服的关键障碍。