Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou.
The Institute of Cardiovascular Sciences and Institute of Systems Biomedicine, School of Basic Medical Sciences, and Key Laboratory of Molecular Cardiovascular Sciences of Ministry of Education, Peking University Health Science Center.
Int J Nanomedicine. 2016 Dec 13;11:6743-6751. doi: 10.2147/IJN.S119607. eCollection 2016.
Drag-reducing polymers (DRPs), when added in minute concentrations, have been shown to decrease peripheral vascular resistance. In this study, the effect of DRPs on the hypertension-induced left ventricular hypertrophy and aortic remodeling was evaluated in spontaneously hypertensive rats (SHR). Male SHR and age-matched Wistar rats were divided into four groups and received intravenous injection of normal saline (NS) or DRPs. Body weight (BW), heart rate (HR) and systolic blood pressure (SBP) were measured. Echocardiography was used to evaluate the changes in left ventricle (LV) function and global wall motion. The LV and aorta were stained by hematoxylin and eosin. Cell size of cardiomyocytes and aortic medial thickness were evaluated for each section. The expression of endothelin-1 (ET-1) of LV and aorta was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry. There was no significant difference in the increase of SBP among SHR + NS, SHR + 10DRP and SHR + 20DRP groups. SHR + NS group had markedly smaller left ventricular end-systolic diameter and left ventricular end-diastolic diameter but bigger anterior and posterior systolic wall thicknesses, while there was no significant difference in fractional shortening and ejection fraction. The cross-sectional areas (CSAs) of cardiomyocytes and the medial thickness of the aorta in SHR + 10 (ppm) DRP and SHR + 20 (ppm) DRP groups were significantly reduced compared with SHR + NS group. The expression of ET-1 in SHR + 10DRP and SHR + 20DRP groups was significantly attenuated. These results suggest that chronic treatment with DRPs can protect against left ventricular hypertrophy and aortic remodeling. DRPs may offer a new approach to the treatment of left ventricular hypertrophy and aortic remodeling caused by hypertension.
减阻聚合物（DRPs）在微小浓度下就已被证实可以降低外周血管阻力。在这项研究中，我们评估了 DRPs 对自发性高血压大鼠（SHR）的高血压诱导的左心室肥厚和主动脉重塑的影响。雄性 SHR 和年龄匹配的 Wistar 大鼠被分为四组，分别接受生理盐水（NS）或 DRPs 的静脉注射。测量体重（BW）、心率（HR）和收缩压（SBP）。超声心动图用于评估左心室（LV）功能和整体壁运动的变化。用苏木精和伊红对 LV 和主动脉进行染色。评估每个切片的心肌细胞大小和主动脉中层厚度。通过定量逆转录聚合酶链反应（qRT-PCR）和免疫组织化学检测 LV 和主动脉内皮素-1（ET-1）的表达。在 SHR + NS、SHR + 10DRP 和 SHR + 20DRP 组中，SBP 的增加没有显著差异。SHR + NS 组的左心室收缩末期直径和左心室舒张末期直径明显较小，但前壁和后壁收缩期厚度较大，而缩短分数和射血分数没有显著差异。与 SHR + NS 组相比，SHR + 10（ppm）DRP 和 SHR + 20（ppm）DRP 组的心肌细胞横截面积（CSAs）和主动脉中层厚度明显减少。SHR + 10DRP 和 SHR + 20DRP 组的 ET-1 表达明显减弱。这些结果表明，DRPs 的慢性治疗可以预防左心室肥厚和主动脉重塑。DRPs 可能为治疗高血压引起的左心室肥厚和主动脉重塑提供一种新方法。
