Zhao Jiajing, Yue Yaohan, Xie Yun, Liu Liwen, Cao Fei, Gao Shurong, Wang Yingjue
Department of Traditional Chinese Medicine, Shanghai Putuo People's Hospital, Shanghai 200060, P.R. China.
Exp Ther Med. 2017 Dec;14(6):5395-5400. doi: 10.3892/etm.2017.5218. Epub 2017 Sep 28.
There is still no resolution for arterial remodeling related with hypertension, though hypertension treatment has access to a number of pharmacological agents. The present study aimed at investigating the prevention of Kuan's roots ( Kuan) against in arterial remodeling . Spontaneously hypertensive rats (SHRs) were intragastrically administered 3, 6 or 12 g/kg Kuan or normal saline or enalapril (2.5 mg/kg) once a day for 8 weeks. Hematoxylin and eosin were used to measure blood pressure and stain carotid and arota. The serum concentration of nitric oxide (NO) was measured by NO assay kit (nitrate reductase method). The endothelin-1 transcriptional level, endothelial NO synthase of endothelium as well as angiotensin II receptor type 1 (AT1R) of aorta and carotid was tested by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and the protein level in aorta was also measured by western blotting. The blood pressure in SHR+enalapril, SHR+3 g/kg, SHR+6 g/kg and SHR+12 g/kg Kuan groups was significantly decreased at 4, 6 and 8 weeks post-treatment compared with SHR group. Different doses of Kuan and enalapril treatment showed aortic wall thinness and strengthened NO serum level, but made no impact on the transcriptional level of AT1R in aorta or endothelial NO synthase in carotid. It is suggested by such results that therapy by Kuan is able to fight against arterial remodeling, thus may provide a new means to treat arterial remodeling caused by hypertension.
尽管高血压治疗有多种药物可用，但与高血压相关的动脉重塑问题仍未得到解决。本研究旨在探讨宽根（宽）对动脉重塑的预防作用。将自发性高血压大鼠（SHR）每天一次灌胃给予3、6或12 g/kg宽根或生理盐水或依那普利（2.5 mg/kg），持续8周。用苏木精和伊红测量血压并对颈动脉和主动脉进行染色。用一氧化氮检测试剂盒（硝酸还原酶法）测量血清一氧化氮（NO）浓度。通过定量逆转录-聚合酶链反应（qRT-PCR）检测主动脉和颈动脉的内皮素-1转录水平、内皮型一氧化氮合酶以及血管紧张素II 1型受体（AT1R），并用蛋白质印迹法测量主动脉中的蛋白质水平。与SHR组相比，SHR+依那普利、SHR+3 g/kg、SHR+6 g/kg和SHR+12 g/kg宽根组在治疗后4、6和8周时血压显著降低。不同剂量的宽根和依那普利治疗显示主动脉壁变薄，血清NO水平升高，但对主动脉中AT1R的转录水平或颈动脉中的内皮型一氧化氮合酶没有影响。这些结果表明，宽根治疗能够对抗动脉重塑，从而可能为治疗高血压引起的动脉重塑提供一种新方法。
