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使用两点校准在CCD Magic Lite免疫分析系统上扩展标准曲线稳定性。

Extended standard curve stability on the CCD Magic Lite immunoassay system using a two-point adjustment.

作者信息

Lynch M J

机构信息

Ciba-Corning Diagnostics, East Walpole, MA 02032.

出版信息

J Biolumin Chemilumin. 1989 Jul;4(1):615-9. doi: 10.1002/bio.1170040181.

Abstract

Ciba Corning Diagnostics has developed a two-point adjustment algorithm for use with the Magic Lite System which allows for extended stability of a full 7-10 point calibration curve over the life of a kit. This adjustment is accomplished by using a master calibration curve established during manufacturing along with two calibrators for each assay. Most conventional non-automated immunoassays contain anywhere from 6 to 10 calibrators which are included with each run of an assay. Eliminating the need to run full standard curves and using the two-point adjustment algorithm results in significant savings in both labour and reagent usage.

摘要

西巴·科宁诊断公司已为Magic Lite系统开发了一种两点校准算法,该算法可使试剂盒在其整个有效期内实现完整的7至10点校准曲线的长期稳定性。这种校准是通过使用制造过程中建立的主校准曲线以及每种检测的两个校准品来完成的。大多数传统的非自动化免疫检测每次检测运行时包含6至10个校准品。无需绘制完整的标准曲线并使用两点校准算法,可显著节省劳动力和试剂用量。

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