Tabassum Heena, Ashafaq Mohammad, Parvez Suhel, Raisuddin Sheikh
Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi 110 062, India.
Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi 110 062, India.
Neurochem Int. 2017 Mar;104:11-26. doi: 10.1016/j.neuint.2016.12.010. Epub 2016 Dec 22.
Nonylphenol (NP), an environmental endocrine disruptor mimics estrogen and is a potential toxicant both under in vitro and in vivo conditions. In this study, the effect of melatonin on NP- induced neurotoxicity and cognitive alteration was investigated in adult male Wistar rats. Melatonin supplementation has been known to protect cells from neurotoxic injury. The animals were divided into three groups namely, control (vehicle) which received olive oil orally and treated rats received NP (25 mg/kg, per os) thrice a week for 45 days while the third group i.e., NP + melatonin, animals were co-administered melatonin (10 mg/kg, i.p.) along with NP. On the 46th day, rats were assessed for anxiety, motor co-ordination, grip strength and cognitive performance using Morris water maze test and then sacrificed for biochemical and histopathological assays in brain tissues. Melatonin improved the behavioral performance in NP exposed group. The results showed that NP significantly decreased the activity of acetylcholine esterase (AchE), monoamine oxidase (MAO) and Na/K-ATPase, in rat brain tissue along with other enzymes of antioxidant milieu. The outcome of the study shows that NP, like other persistent endocrine disrupting pollutants, creates a potential risk of cognitive, neurochemical and histopathological perturbations as a result of environmental exposure. Taken together, our study demonstrates that melatonin is protective against NP-induced neurotoxicity.
壬基酚（NP）是一种环境内分泌干扰物，可模拟雌激素，在体外和体内条件下均为潜在毒物。在本研究中，研究了褪黑素对成年雄性Wistar大鼠NP诱导的神经毒性和认知改变的影响。已知补充褪黑素可保护细胞免受神经毒性损伤。将动物分为三组，即对照组（赋形剂），口服橄榄油；处理组大鼠每周三次接受NP（25mg/kg，经口），共45天；第三组即NP+褪黑素组，动物在接受NP的同时腹腔注射褪黑素（10mg/kg）。在第46天，使用莫里斯水迷宫试验评估大鼠的焦虑、运动协调性、握力和认知表现，然后处死大鼠以进行脑组织的生化和组织病理学分析。褪黑素改善了NP暴露组的行为表现。结果表明，NP显著降低了大鼠脑组织中乙酰胆碱酯酶（AchE）、单胺氧化酶（MAO）和钠钾ATP酶（Na/K-ATPase）的活性以及抗氧化环境中的其他酶活性。研究结果表明，NP与其他持久性内分泌干扰污染物一样，由于环境暴露会产生认知、神经化学和组织病理学紊乱的潜在风险。综上所述，我们的研究表明褪黑素对NP诱导的神经毒性具有保护作用。
