Kearney Brian J, Voorhees Matthew A, Williams Priscilla L, Olschner Scott P, Rossi Cynthia A, Schoepp Randal J
Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, MD, 21702, United States.
Ke'aki Technologies, LLC, 1425 Porter Street, Fort Detrick, MD, 21702, United States.
J Virol Methods. 2017 Apr;242:9-13. doi: 10.1016/j.jviromet.2016.12.011. Epub 2016 Dec 21.
Viral preparations are essential components in diagnostic research and development. The production of large quantities of virus traditionally is done by infecting numerous tissue culture flasks or roller bottles, which require large incubators and/or roller bottle racks. The Corning HYPERFlask is a multilayer flask that uses a gas permeable film to provide gas exchange between the cells and culture medium and the atmospheric environment. This study evaluated the suitability of the HYPERFlask for production of Lassa, Ebola, Bundibugyo, Reston, and Marburg viruses and compared it to more traditional methods using tissue culture flasks and roller bottles. The HYPERFlask produced cultures were equivalent in virus titer and indistinguishable in immunodiagnostic assays. The use of the Corning HYPERFlask for viral production is a viable alternative to traditional tissue culture flasks and roller bottles. HYPERFlasks allow for large volumes of virus to be produced in a small space without specialized equipment.
病毒制剂是诊断研究与开发中的重要组成部分。传统上，大量病毒的生产是通过感染众多的组织培养瓶或滚瓶来完成的，这需要大型培养箱和/或滚瓶架。康宁HYPERFlask是一种多层培养瓶，它使用透气膜在细胞与培养基以及大气环境之间进行气体交换。本研究评估了HYPERFlask用于生产拉沙病毒、埃博拉病毒、本迪布焦病毒、雷斯顿病毒和马尔堡病毒的适用性，并将其与使用组织培养瓶和滚瓶的更传统方法进行了比较。使用HYPERFlask生产的培养物在病毒滴度上相当，在免疫诊断试验中无法区分。使用康宁HYPERFlask进行病毒生产是传统组织培养瓶和滚瓶的可行替代方法。HYPERFlask能够在小空间内生产大量病毒，无需专门设备。
