Bao Xuerui, Jia Xiangyin, Chen Lequn, Peters Brian M, Lin Chii-Wann, Chen Dingqiang, Li Lin, Li Bing, Li Yanyan, Xu Zhenbo, Shirtliff Mark E
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
Microb Pathog. 2017 May;106:16-19. doi: 10.1016/j.micpath.2016.12.012. Epub 2016 Dec 22.
Cronobacter sakazakii (C.sakazakii) has been identified as a wide-spread conditioned pathogen associated with series of serious illnesses, such as neonatal meningitis, enterocolitis, bacteremia or sepsis. As food safety is concerned, microbial biofilm has been considered to be a potential source of food contamination. The current study aims to investigate the ability of biofilm formation of two C. sakazakii strains (wild type BAA 894 and pmrA mutant). Crystal violet (CV), XTT (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino carbonyl)-2H-(tetrazolium hydroxide)] assays, and scanning electron microscopy (SEM) are performed on different time points during biofilm formation of C. sakazakii strains. Furthermore, RNA-seq strategy is utilized and the transcriptome data is analyzed to study the expression of genes related to biofilm formation along with whole genome sequencing. For biomass, in the first 24 h, pmrA mutant produced approximately 5 times than wildtype. However, the wild type exhibited more biomass than pmrA mutant during the post maturation stage (7-14 d). In addition, the wildtype showed higher viability than pmrA mutant during the whole biofilm formation. This study represents the first evidence on the biofilm formation of C. sakazakii pmrA mutant, which may further aid in the prevention and control for the food contamination caused by C. sakazakii.
阪崎肠杆菌已被确认为一种广泛存在的条件致病菌，与一系列严重疾病有关，如新生儿脑膜炎、小肠结肠炎、菌血症或败血症。就食品安全而言，微生物生物膜被认为是食品污染的一个潜在来源。本研究旨在调查两株阪崎肠杆菌（野生型BAA 894和pmrA突变体）形成生物膜的能力。在阪崎肠杆菌菌株生物膜形成的不同时间点进行结晶紫（CV）、XTT（2-甲氧基-4-硝基-5-磺基苯基）-5-[(苯基氨基羰基)-2H-(四唑氢氧化合物)]测定以及扫描电子显微镜（SEM）观察。此外，利用RNA测序策略并分析转录组数据，以研究与生物膜形成相关基因的表达以及全基因组测序。对于生物量，在最初的24小时内，pmrA突变体产生的生物量约为野生型的5倍。然而，在成熟后期（7-14天），野生型的生物量比pmrA突变体更多。此外，在整个生物膜形成过程中，野生型的活力高于pmrA突变体。本研究首次提供了阪崎肠杆菌pmrA突变体生物膜形成的证据，这可能有助于进一步预防和控制阪崎肠杆菌引起的食品污染。
