Department of Haematology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510623, China.
Department of Spine Surgery, The Second Affiliated Hospital of Shantou University Medical College, North Dongxia Road, Shantou, 515041, Guangdong, China.
Bioprocess Biosyst Eng. 2021 May;44(5):985-994. doi: 10.1007/s00449-020-02449-8. Epub 2020 Oct 28.
Staphylococcus aureus (S. aureus) is an important human pathogen causing a variety of life-threatening diseases. In recent years, the health problem caused by S. aureus contaminated food has become a global health problem. S. aureus can express various pathogenic factors, mainly used for adhesion, colonization, invasion and infection of the host. Therefore, rapid and accurate detection of virulence genes in S. aureus is necessary to prevent outbreaks caused by this pathogen. PCR is a useful tool for rapid detection of foodborne pathogens. The objective of this study was to detect the presence of major toxin genes in S. aureus, including sea, seb, sec, see, pvl and tsst, by using a PCR plate. Of the 13 strains tested, 12 (92.3%) were found to be positive for one or more toxin genes. This study realized the one-step detection of main toxin factors in S. aureus.
金黄色葡萄球菌（S. aureus）是一种重要的人类病原体，可引起多种危及生命的疾病。近年来，由金黄色葡萄球菌污染食物引起的健康问题已成为全球性健康问题。金黄色葡萄球菌可以表达各种致病因子，主要用于宿主的黏附、定植、侵袭和感染。因此，快速准确地检测金黄色葡萄球菌中的毒力基因对于预防这种病原体引起的暴发是必要的。PCR 是一种快速检测食源性病原体的有用工具。本研究旨在通过 PCR 板检测金黄色葡萄球菌中主要毒素基因 sea、seb、sec、see、pvl 和 tsst 的存在。在检测的 13 株菌株中，有 12 株（92.3%）被发现携带一种或多种毒素基因。本研究实现了金黄色葡萄球菌主要毒素因子的一步检测。
