Klein H H, Pich S, Lindert S, Nebendahl K, Niedmann P, Kreuzer H
Department of Cardiology, University of Göttingen, West Germany.
Am Heart J. 1989 Oct;118(4):667-73. doi: 10.1016/0002-8703(89)90577-2.
The effect of two different combined treatments with vitamin E acetate and vitamin C on infarct size and recovery of regional myocardial function was investigated in ischemic, reperfused porcine hearts. The left anterior descending coronary artery was distally ligated in 30 thoracotomized pigs for 45 minutes followed by 3 days of reperfusion. Infarct size was determined as the ratio of infarcted (tetrazolium stain) to ischemic (dye technique) myocardium. Regional myocardial function was assessed by sonomicrometry. Ten pigs received vitamin E acetate (12 gm intravenously three times for 1 week) before ischemic and vitamin C (4.4 gm intravenously) before reperfusion (therapy A). Another 10 pigs were treated with vitamin E acetate (12 gm intraarterially) and vitamin C (4.4 gm intravenously) during ischemia (therapy B). An additional 10 pigs served as a control group. Global hemodynamics did not differ significantly among the groups before and during ischemia. Mean plasma concentrations of vitamin E amounted to 107 micrograms/ml in group A, 16 micrograms/ml in group B, and 0.9 micrograms/ml in the control group at the onset of reperfusion. Therapy A reduced the size of the infarct from 73 +/- 12% to 47 +/- 16% of the region at risk (p less than 0.005) and improved regional systolic shortening from 0 +/- 7% to 11 +/- 6% at 3 days after reperfusion (p less than 0.01). Therapy B decreased the size of the infarct to 64 +/- 9% of the region at risk (p = 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
在缺血再灌注的猪心脏中，研究了维生素E醋酸酯和维生素C两种不同联合治疗对梗死面积和局部心肌功能恢复的影响。对30只开胸猪的左前降支冠状动脉进行远端结扎45分钟，随后再灌注3天。梗死面积通过梗死心肌（四氮唑染色）与缺血心肌（染料技术）的比例来确定。局部心肌功能通过超声微测法评估。10只猪在缺血前接受维生素E醋酸酯（静脉注射12克，每周3次，共1周），再灌注前接受维生素C（静脉注射4.4克）（治疗A）。另外10只猪在缺血期间接受维生素E醋酸酯（动脉内注射12克）和维生素C（静脉注射4.4克）（治疗B）。另有10只猪作为对照组。各组在缺血前和缺血期间的整体血流动力学无显著差异。再灌注开始时，治疗A组维生素E的平均血浆浓度为107微克/毫升，治疗B组为16微克/毫升，对照组为0.9微克/毫升。治疗A使梗死面积从危险区域的73±12%降至47±16%（p<0.005），并使再灌注3天后局部收缩期缩短率从0±7%提高到11±6%（p<0.01）。治疗B使梗死面积降至危险区域的64±9%（p = 0.05）。（摘要截断于250字）
