Pang Yuxuan, Guan Lin, Zhu Yanlin, Niu Ruijuan, Zhu Song, Lin Quan
Department of Prosthodontics, School and Hospital of Stomatology, Jilin University, Changchun, China.
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, China.
Front Bioeng Biotechnol. 2023 Jun 2;11:1162202. doi: 10.3389/fbioe.2023.1162202. eCollection 2023.
An open critical-size bone defect is a major medical problem because of the difficulty in self-healing, leading to an increased risk of bacterial infection owing to wound exposure, resulting in treatment failure. Herein, a composite hydrogel was synthesized by chitosan, gallic acid, and hyaluronic acid, termed "CGH." Hydroxyapatite was modified with polydopamine (PDA@HAP) and introduced to CGH to obtain a mussel-inspired mineralized hydrogel (CGH/PDA@HAP). The CGH/PDA@HAP hydrogel exhibited excellent mechanical performances, including self-healing and injectable properties. Owing to its three-dimensional porous structure and polydopamine modifications, the cellular affinity of the hydrogel was enhanced. When adding PDA@HAP into CGH, Ca and PO could release and then promoted differentiation of BMSCs into osteoblasts. Without any osteogenic agent or stem cells, the area of new bone at the site of defect was enhanced and the newly formed bone had a dense trabecular structure after implanting of the CGH/PDA@HAP hydrogel for 4 and 8 weeks. Moreover, the growth of and was effectively inhibited through the grafting of gallic acid onto chitosan. Above, this study provides a reasonable alternative strategy to manage open bone defects.
开放性临界尺寸骨缺损是一个重大的医学问题，因为其自我愈合困难，由于伤口暴露导致细菌感染风险增加，进而导致治疗失败。在此，通过壳聚糖、没食子酸和透明质酸合成了一种复合水凝胶，称为“CGH”。用聚多巴胺修饰羟基磷灰石（PDA@HAP）并将其引入CGH中，以获得一种受贻贝启发的矿化水凝胶（CGH/PDA@HAP）。CGH/PDA@HAP水凝胶表现出优异的力学性能，包括自我愈合和可注射性能。由于其三维多孔结构和聚多巴胺修饰，水凝胶的细胞亲和力得到增强。当将PDA@HAP添加到CGH中时，Ca和PO可以释放，然后促进骨髓间充质干细胞向成骨细胞分化。在没有任何成骨剂或干细胞的情况下，植入CGH/PDA@HAP水凝胶4周和8周后，缺损部位的新骨面积增加，新形成的骨具有致密的小梁结构。此外，通过将没食子酸接枝到壳聚糖上，有效地抑制了 和 的生长。综上所述，本研究为处理开放性骨缺损提供了一种合理的替代策略。
