Géloën A, Roy P E, Bukowiecki L J
Department of Physiology, Medical School, Laval University, Quebec, Canada.
Am J Physiol. 1989 Oct;257(4 Pt 1):E547-53. doi: 10.1152/ajpendo.1989.257.4.E547.
The effects of long-term diabetes (4 wk) on the development of parametrial (PWAT) and retroperitoneal (RWAT) white adipose tissues were studied in young Sprague-Dawley rats (170-200 g) injected with a single dose of streptozotocin (75 mg/kg). Diabetes stopped animal growth and totally abolished the normal increases in the wet weight, total protein content, and cellularity (estimated by DNA content) of PWAT and RWAT. Remarkably, the prolonged lack of insulin induced a progressive decrease of the cellularity of RWAT to levels that were lower than those of the initial controls. It also resulted in a marked reduction of adipocyte size. The tiny adipocytes seen in diabetic animals were characterized by the presence of multilocular triglyceride droplets. In general, the decreases in cell number, cell size, and protein content were more pronounced in RWAT than in PWAT. Quantitative cellular frequency studies revealed that adipocytes, and possibly also endothelial cells, contribute to the decrease in RWAT cellularity. The results demonstrate that 1) diabetes inhibits proliferative activity in adipose tissue, 2) total cell number reduction may occur in adipose depot of young growing rats, 3) this effect is depot dependent, and 4) the turnover of adipocytes and endothelial cells is relatively slow (several weeks).
在注射单剂量链脲佐菌素（75毫克/千克）的年轻斯普拉格-道利大鼠（170 - 200克）中，研究了长期糖尿病（4周）对子宫旁白色脂肪组织（PWAT）和腹膜后白色脂肪组织（RWAT）发育的影响。糖尿病抑制了动物生长，并完全消除了PWAT和RWAT湿重、总蛋白含量及细胞数量（通过DNA含量估算）的正常增加。值得注意的是，长期缺乏胰岛素导致RWAT细胞数量逐渐减少至低于初始对照组的水平。这也导致脂肪细胞大小显著减小。糖尿病动物中可见的微小脂肪细胞的特征是存在多泡状甘油三酯滴。总体而言，RWAT中细胞数量、细胞大小和蛋白质含量的减少比PWAT更明显。定量细胞频率研究表明，脂肪细胞以及可能还有内皮细胞导致了RWAT细胞数量的减少。结果表明：1）糖尿病抑制脂肪组织中的增殖活性；2）年轻生长大鼠的脂肪库中可能会出现总细胞数量减少；3）这种效应具有脂肪库依赖性；4）脂肪细胞和内皮细胞的更新相对较慢（数周）。
