Department of Oral and Maxillofacial Surgery, Division of Clinical Medicine, Graduate Faculty of Interdisciplinary Research, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, Japan.
Depatment of Medical Institure, Takiron Co. Ltd, 7-1-9, Minatojimaminamimachi, Chuo-ku, Kobe, Hyogo, Japan.
J Biomed Mater Res B Appl Biomater. 2018 Jan;106(1):191-200. doi: 10.1002/jbm.b.33833. Epub 2016 Dec 26.
Materials fabricated with unsintered hydroxyapatite/poly-l-lactic acid (uHA/PLLA) exhibit biological activity and biocompatibility, but are also hydrophobic. This hydrophobicity limits the ability of cells or tissues to adhere to the surface of the material and thereby prevents the materials from exhibiting effective biological activity. In this study, we examined the effects of ultraviolet (UV) treatment on the hydrophobicity of uHA/PLLA. We compared the contact angle, mechanical strength, cell attachment, and cell differentiation capacity between untreated uHA/PLLA and uHA/PLLA treated with UV light. The contact angle of UV-treated uHA/PLLA was significantly reduced compared with that of untreated uHA/PLLA. However, there were no differences in mechanical strength between untreated uHA/PLLA and UV-treated uHA/PLLA. Notably, the ability of cells to adhere to UV-treated uHA/PLLA was significantly increased compared with that of untreated uHA/PLLA. Similarly, there were significant differences between UV-treated uHA/PLLA and untreated uHA/PLLA in alkaline phosphatase assays. These findings demonstrated that UV irradiation of uHA/PLLA improved the surface hydrophilicity without changing the mechanical strength of the material; thus, UV treatment of uHA/PLLA may facilitate the use of this material in biomedical applications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 191-200, 2018.
用未烧结的羟基磷灰石/聚左旋乳酸（uHA/PLLA）制造的材料具有生物活性和生物相容性，但也具有疏水性。这种疏水性限制了细胞或组织在材料表面的附着能力，从而阻止了材料表现出有效的生物活性。在这项研究中，我们研究了紫外线（UV）处理对 uHA/PLLA 疏水性的影响。我们比较了未经处理的 uHA/PLLA 和经过 UV 光处理的 uHA/PLLA 的接触角、机械强度、细胞附着和细胞分化能力。与未经处理的 uHA/PLLA 相比，经 UV 处理的 uHA/PLLA 的接触角显著降低。然而，未经处理的 uHA/PLLA 和经 UV 处理的 uHA/PLLA 的机械强度没有差异。值得注意的是，与未经处理的 uHA/PLLA 相比，细胞在经 UV 处理的 uHA/PLLA 上的附着能力显著增加。同样，碱性磷酸酶测定中经 UV 处理的 uHA/PLLA 和未经处理的 uHA/PLLA 之间也存在显著差异。这些发现表明，uHA/PLLA 的 UV 照射提高了表面亲水性，而不改变材料的机械强度；因此，uHA/PLLA 的 UV 处理可能有助于该材料在生物医学应用中的使用。© 2016 Wiley Periodicals, Inc. J 生物医学材料研究杂志 B：应用生物材料，106B：191-200，2018。
