Jin Yaqing, Zhang Nengpan, Li Chunlin, Pu Kefeng, Ding Chen, Zhu Yimin
Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Dushu Lake Higher Education Town, Suzhou Industrial Park, Suzhou, 215125, China; University of Chinese Academy of Sciences, 19(A) Yuquan Road, Beijing, 100039, China.
Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Dushu Lake Higher Education Town, Suzhou Industrial Park, Suzhou, 215125, China.
Colloids Surf B Biointerfaces. 2017 Mar 1;151:240-248. doi: 10.1016/j.colsurfb.2016.12.024. Epub 2016 Dec 20.
A dual-functional delivery system, based on mesoporous silica nanoparticles (MSNs) with the integration of Magnetic Resonance (MR) imaging and therapeutic peptide delivery, is reported in this paper. A lipid bilayer is attached onto the surface of the nanoparticles, following the doping of Gadolinium (Gd), a paramagnetic lanthanide ion. The liposome-coated GdMSNs exhibit improved colloidal stability, better biocompatibility and more efficient cellular uptake. The Gd renders the nano carrier a potential T1 contrast agent, confirmed by the MR imaging. A pro-apoptotic peptide, KLA (HGGKLAKLAKKLAKLAK), is encapsulated into the GdMSNs-LP and enters into the cells successfully to induce mitochondrial swelling and apoptosis, while it is nontoxic outside the cells. The synthesis procedure is convenient and free of toxic organic reagents. The nanosystem we construct may contribute to a promising theranostic platform for therapeutic peptide delivery in cancer treatment.
本文报道了一种基于介孔二氧化硅纳米颗粒（MSNs）的双功能递送系统，该系统集成了磁共振（MR）成像和治疗性肽递送功能。在掺杂顺磁性镧系离子钆（Gd）之后，脂质双层附着在纳米颗粒表面。脂质体包被的GdMSNs表现出更好的胶体稳定性、生物相容性以及更高的细胞摄取效率。通过MR成像证实，Gd使纳米载体成为一种潜在的T1造影剂。一种促凋亡肽KLA（HGGKLAKLAKKLAKLAK）被封装到GdMSNs-LP中，并成功进入细胞诱导线粒体肿胀和凋亡，而在细胞外它是无毒的。合成过程简便且无需使用有毒有机试剂。我们构建的纳米系统可能为癌症治疗中的治疗性肽递送提供一个有前景的诊疗平台。
