Ravagnani F, Siena S, De Reys S, Di Nicola M, Notti P, Giardini R, Bregni M, Matteucci P, Gianni A M, Pellegris G
Division of Immunohematology and Medical Oncology, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy.
Transfusion. 1999 Jan;39(1):48-55. doi: 10.1046/j.1537-2995.1999.39199116894.x.
For simplification of blood cell transplantation, an automated apheresis system that exploits a dual-stage channel device for mononuclear cell (MNC) collection (AutoPBSC, designed for the COBE Spectra) was studied.
The automated default software (AutoPBSC-Default) and three software modifications of the harvest frequency during leukapheresis, referred to as AutoPBSC-1.25, AutoPBSC-1.75, and AutoPBSC-2.75, were evaluated in comparison with the semiautomated Version 4.7 (V4.7) apheresis system in 119 leukapheresis procedures performed in 90 cancer patients treated with chemotherapy plus granulocyte-colony-stimulating factor. CD34+ cell and platelet collection efficiency (CE); volume and cell composition of the leukapheresis components; and patient platelet and red cell (RBC) loss during leukapheresis were measured.
The majority of collection measures evaluated with the AutoPBSC compared favorably to those obtained with the V4.7. CD34+ cell CE increased from 55 percent with V4.7 to 68 percent with the AutoPBSC-Default (p = 0.05). The AutoPBSC provided lower platelet contamination in the collected component (1.18 x 10(11) vs. 2.26 x 10(11) with the V4.7; p<0.001). The volume of the AutoPBSC-Default component was significantly lower (67 vs. 180 mL with the V4.7; p<0.001). The MNC purity of the AutoPBSC component was greater (52 vs. 28% with the V4.7; p<0.001), and the RBC contamination lower (AutoPBSC, 0.53 x 10(11) vs. 1.04 x 10(11) with the V4.7; p<0.001). Modifications of the AutoPBSC to increase the harvest frequency by 1.25-, 1.75-, and 2.75-fold resulted in increased CD34+ cell CE (77%, 75%, and 83%, respectively; p<0.001 in all cases), but also in reduced numbers of circulating platelets, higher platelet contamination of the component, and lower MNC purity than were seen with the AutoPBSC-Default.
The AutoPBSC offers the following advantages over the V4.7 system: a) better CE of CD34+ cells; b) reduced collection of platelets; c) reduced contamination of the leukapheresis component with granulocytes, platelets, and RBCs; d) reduced component volume; and e) automation.
为简化血细胞移植，研究了一种利用双阶段通道装置采集单核细胞（MNC）的自动单采系统（专为COBE Spectra设计的AutoPBSC）。
在90例接受化疗加粒细胞集落刺激因子治疗的癌症患者进行的119次白细胞单采过程中，将自动默认软件（AutoPBSC-Default）以及白细胞单采过程中采集频率的三种软件修改版本（分别称为AutoPBSC-1.25、AutoPBSC-1.75和AutoPBSC-2.75）与半自动4.7版（V4.7）单采系统进行比较。测量了CD34+细胞和血小板采集效率（CE）；白细胞单采成分的体积和细胞组成；以及白细胞单采过程中患者血小板和红细胞（RBC）的损失。
与V4.7相比，使用AutoPBSC评估的大多数采集指标表现更优。CD34+细胞CE从V4.7的55%提高到AutoPBSC-Default的68%（p = 0.05）。AutoPBSC采集成分中的血小板污染更低（1.18×10¹¹ 对比V4.7的2.26×10¹¹；p<0.001）。AutoPBSC-Default成分的体积显著更低（67对比V4.7的180 mL；p<0.001）。AutoPBSC成分的MNC纯度更高（52%对比V4.7的28%；p<0.001），RBC污染更低（AutoPBSC为0.53×10¹¹ 对比V4.7的1.04×10¹¹；p<0.001）。将AutoPBSC的采集频率提高1.25倍、1.75倍和2.75倍的修改导致CD34+细胞CE增加（分别为77%、75%和83%；所有情况p<0.001），但也导致循环血小板数量减少、成分中血小板污染更高以及MNC纯度低于AutoPBSC-Default。
与V4.7系统相比，AutoPBSC具有以下优势：a）CD34+细胞的CE更好；b）血小板采集减少；c）白细胞单采成分中粒细胞、血小板和RBC的污染减少；d）成分体积减小；以及e）自动化。
