Gabriel Laís P, Rodrigues Ana Amélia, Macedo Milton, Jardini André L, Maciel Filho Rubens
National Institute of Biofabrication, Campinas, Brazil; Department of Chemical Engineering, University of Campinas, Campinas, Brazil.
National Institute of Biofabrication, Campinas, Brazil; Department of Medical Sciences, University of Campinas, Campinas, Brazil.
Mater Sci Eng C Mater Biol Appl. 2017 Mar 1;72:113-117. doi: 10.1016/j.msec.2016.11.057. Epub 2016 Nov 16.
Tissue Engineering proposes, among other things, tissue regeneration using scaffolds integrated with biological molecules, growth factors or cells for such regeneration. In this research, polyurethane membranes were prepared using the electrospinning technique in order to obtain membranes to be applied in Tissue Engineering, such as epithelial, drug delivery or cardiac applications. The influence of fibers on the structure and morphology of the membranes was studied using scanning electron microscopy (SEM), the structure was evaluated by Fourier transform infrared spectroscopy (FT-IR), and the thermal stability was analyzed by thermogravimetry analysis (TGA). In vitro cells attachment and proliferation was investigated by SEM, and in vitro cell viability was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays and Live/Dead® assays. It was found that the membranes present an homogeneous morphology, high porosity, high surface area/volume ratio, it was also observed a random fiber network. The thermal analysis showed that the membrane degradation started at 254°C. In vitro evaluation of fibroblasts cells showed that fibroblasts spread over the membrane surface after 24, 48 and 72h of culture. This study supports the investigation of electrospun polyurethane membranes as biocompatible scaffolds for Tissue Engineering applications and provides some guidelines for improved biomaterials with desired properties.
组织工程学提出了多种方法，其中包括使用与生物分子、生长因子或细胞相结合的支架来实现组织再生。在本研究中，采用静电纺丝技术制备了聚氨酯膜，以获得可应用于组织工程的膜，如上皮组织、药物递送或心脏应用等。使用扫描电子显微镜（SEM）研究了纤维对膜结构和形态的影响，通过傅里叶变换红外光谱（FT-IR）评估了结构，并通过热重分析（TGA）分析了热稳定性。通过SEM研究了体外细胞的附着和增殖情况，并通过3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-溴化四氮唑（MTT）法和活/死®法研究了体外细胞活力。结果发现，这些膜呈现出均匀的形态、高孔隙率、高表面积/体积比，还观察到了随机的纤维网络。热分析表明，膜的降解始于254°C。对成纤维细胞的体外评估表明，在培养24、48和72小时后，成纤维细胞在膜表面扩散。本研究支持将静电纺丝聚氨酯膜作为组织工程应用的生物相容性支架进行研究，并为改进具有所需性能的生物材料提供了一些指导方针。
