Nirmala R, Kang Hyung-Sub, El-Newehy Mohamed H, Navamathavan R, Park Hye-Min, Kim Hak Yong
Bio-Nano System Engineering, College of Engineering, Chonbuk National University, Jeonju 561 756, South Korea.
J Nanosci Nanotechnol. 2011 Jun;11(6):4749-56. doi: 10.1166/jnn.2011.3883.
This work was focused on preparation and characterization of the polyurethane nanofibers containing calcium chloride (CaCl2) prepared via electrospinning process for the bionanotechnological applications. The morphological, structural characterizations and thermal properties of the polyurethane/CaCl2 nanofibers were determined by using scanning electron microscopy (SEM), field-emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX), Fourier transform-infrared (FT-IR) spectroscopy and thermogravimetry (TGA). SEM images revealed that these composite nanofibers were well-oriented and had good incorporation of CaCl2. The morphological feature of the cells attached on polyurethane/CaCl2 nanofibers scaffold was confirmed by SEM. The in vitro cell compatibility of the polyurethane/CaCl2 composite nanofibers was studied. This study demonstrated the non-toxic effect of electrospun polyurethane/CaCl2 composite nanofibers. Based on this, we propose a mechanism for the cell attachment.
本研究聚焦于通过静电纺丝工艺制备含氯化钙（CaCl2）的聚氨酯纳米纤维，并对其进行表征，以用于生物纳米技术应用。采用扫描电子显微镜（SEM）、场发射扫描电子显微镜（FE-SEM）、能量色散X射线光谱（EDX）、傅里叶变换红外（FT-IR）光谱和热重分析（TGA）来测定聚氨酯/CaCl2纳米纤维的形态、结构特征和热性能。SEM图像显示这些复合纳米纤维排列良好，且CaCl2掺入效果良好。通过SEM证实了附着在聚氨酯/CaCl2纳米纤维支架上细胞的形态特征。研究了聚氨酯/CaCl2复合纳米纤维的体外细胞相容性。本研究证明了静电纺丝聚氨酯/CaCl2复合纳米纤维的无毒作用。基于此，我们提出了一种细胞附着机制。
