Cho Youngchae, Jeong Heeseok, Kim Baeyeon, Jang Juwoong, Song Yo-Seung, Lee Deuk Yong
Department of Biomedical Engineering, Daelim University, Anyang 13916, Republic of Korea.
Department of Materials Science and Engineering, Incheon National University, Incheon 22012, Republic of Korea.
Polymers (Basel). 2023 Sep 21;15(18):3844. doi: 10.3390/polym15183844.
Poly(L-lactic acid) (PLLA) and PLLA/gelatin polymers were prepared via electrospinning to evaluate the effect of PLLA and gelatin content on the mechanical properties, water uptake capacity (WUC), water contact angle (WCA), degradation rate, cytotoxicity and cell proliferation of membranes. As the PLLA concentration increased from 1 wt% to 3 wt%, the tensile strength increased from 5.8 MPa to 9.1 MPa but decreased to 7.0 MPa with 4 wt% PLLA doping. The WUC decreased rapidly from 594% to 236% as the PLLA content increased from 1 to 4 wt% due to the increased hydrophobicity of PLLA. As the gelatin content was increased to 3 wt% PLLA, the strength, WUC and WCA of the PLLA/gelatin membrane changed from 9.1 ± 0.9 MPa to 13.3 ± 2.3 MPa, from 329% to 1248% and from 127 ± 1.2° to 0°, respectively, with increasing gelatin content from 0 to 40 wt%. However, the failure strain decreased from 3.0 to 0.5. The biodegradability of the PLLA/gelatin blend increased from 3 to 38% as the gelatin content increased to 40 wt%. The viability of L-929 and MG-63 cells in the PLLA/gelatin blend was over 95%, and the excellent cell proliferation and mechanical properties suggested that the tunable PLLA/gelatin barrier membrane was well suited for absorbable periodontal tissue regeneration.
通过静电纺丝制备了聚（L-乳酸）（PLLA）和PLLA/明胶聚合物，以评估PLLA和明胶含量对膜的机械性能、吸水能力（WUC）、水接触角（WCA）、降解速率、细胞毒性和细胞增殖的影响。随着PLLA浓度从1 wt%增加到3 wt%，拉伸强度从5.8 MPa增加到9.1 MPa，但在掺杂4 wt% PLLA时降至7.0 MPa。随着PLLA含量从1 wt%增加到4 wt%，由于PLLA疏水性增加，WUC从594%迅速降至236%。随着明胶含量增加到3 wt% PLLA，随着明胶含量从o wt%增加到40 wt%，PLLA/明胶膜的强度、WUC和WCA分别从9.1±0.9 MPa变为13.3±2.3 MPa，从329%变为1248%，从127±1.2°变为0°。然而，断裂应变从3.0降至0.5。随着明胶含量增加到40 wt%，PLLA/明胶共混物的生物降解性从3%增加到38%。PLLA/明胶共混物中L-929和MG-63细胞的活力超过95%，优异的细胞增殖和机械性能表明，可调节的PLLA/明胶屏障膜非常适合可吸收牙周组织再生。
