Li Xiufang, Liu Yan, Zhang Jian, You Renchuan, Qu Jing, Li Mingzhong
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
Department of Burn and Plastic Surgery, School of Medicine Affiliated Ruijin Hospital, Shanghai JiaoTong University, Shanghai 200025, China.
Mater Sci Eng C Mater Biol Appl. 2017 Mar 1;72:394-404. doi: 10.1016/j.msec.2016.11.085. Epub 2016 Nov 24.
The healing of chronic wounds remains a key challenge in regenerative medicine. To promote wound healing, a bioactive wound dressing is required. In this study, a functionalized silk fibroin dressing with topical bioactive insulin release was prepared for the treatment of chronic wounds. For this purpose, insulin-encapsulated silk fibroin (SF) microparticles were prepared by coaxial electrospraying of aqueous SF solution under mild processing conditions. Insulin was successfully encapsulated in the inner layer of SF microparticles, providing a sustained insulin release for up to 28days. It was found that the insulin released from the microparticles could maintain original molecular conformation. Moreover, the cell migration assay based on human keratinocyte and endothelial cells confirmed that the insulin released from SF microparticles retained its native bioactivity. Furthermore, the insulin-encapsulated microparticles were loaded into a SF sponge, functioned as a bioactive wound dressing, and the in vivo therapeutic effect of the sponge dressing was evaluated on dorsal full thickness wounds of diabetic Sprague-Dawley rats. The results showed that an insulin-functionalized SF dressing accelerated wound closure, collagen deposition and vascularization, thus, significantly promoting wound healing. The insulin-functionalized SF dressing provides new treatment options for chronic wounds.
慢性伤口的愈合仍然是再生医学中的一项关键挑战。为促进伤口愈合，需要一种生物活性伤口敷料。在本研究中，制备了一种具有局部生物活性胰岛素释放功能的丝素蛋白敷料，用于治疗慢性伤口。为此，在温和的加工条件下，通过同轴电喷雾SF水溶液制备了包裹胰岛素的丝素蛋白（SF）微粒。胰岛素成功包裹在SF微粒的内层，实现了长达28天的胰岛素持续释放。研究发现，从微粒中释放的胰岛素能够保持原始分子构象。此外，基于人角质形成细胞和内皮细胞的细胞迁移试验证实，从SF微粒中释放的胰岛素保留了其天然生物活性。此外，将包裹胰岛素的微粒加载到SF海绵中，制成生物活性伤口敷料，并在糖尿病斯普拉格-道利大鼠的背部全层伤口上评估了海绵敷料的体内治疗效果。结果表明，胰岛素功能化的SF敷料加速了伤口闭合、胶原蛋白沉积和血管生成，从而显著促进了伤口愈合。胰岛素功能化的SF敷料为慢性伤口提供了新的治疗选择。
