Eslami-Behroozi Mehdi, Pazhoohan Saeed, Aref Ehsan, Zare Leila, Javan Mohammad, Hajizadeh Sohrab, Raoufy Mohammad Reza
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Nasr Bridge, Jalal Al Ahmad Highway, Tehran, Iran.
Chronic Respiratory Diseases Research Center (CRDRC), National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Lung. 2017 Apr;195(2):167-171. doi: 10.1007/s00408-016-9970-4. Epub 2016 Dec 26.
The impact of mechanical forces on pathogenesis of airway remodeling and the functional consequences in asthma remains to be fully established. In the present study, we investigated the effect of repeated bronchoconstriction induced by methacholine (MCh) on airway remodeling and airway hyperresponsiveness (AHR) in rats with or without sensitization to an external allergen. We provide evidence that repeated bronchoconstriction, using MCh, alone induces airway inflammation and remodeling as well as AHR in non-allergen-sensitized rats. Also, we found that the airways are structurally and functionally altered by bronchoconstriction induced by either allergen or MCh in allergen-sensitized animals. This finding provides a new animal model for the development of airway remodeling and AHR in mammals and can be used for studying the complex reciprocal relationship between bronchoconstriction and airway inflammation. Further studies on presented animal models are required to clarify the exact mechanisms underlying airway remodeling due to bronchoconstriction and the functional consequences.
机械力对气道重塑发病机制的影响以及在哮喘中的功能后果仍有待充分明确。在本研究中，我们调查了在对外部过敏原致敏或未致敏的大鼠中，由乙酰甲胆碱（MCh）诱导的反复支气管收缩对气道重塑和气道高反应性（AHR）的影响。我们提供的证据表明，单独使用MCh进行反复支气管收缩可在非过敏原致敏大鼠中诱导气道炎症、重塑以及AHR。此外，我们发现，在过敏原致敏动物中，由过敏原或MCh诱导的支气管收缩会使气道在结构和功能上发生改变。这一发现为哺乳动物气道重塑和AHR的发展提供了一种新的动物模型，可用于研究支气管收缩与气道炎症之间复杂的相互关系。需要对所呈现的动物模型进行进一步研究，以阐明支气管收缩导致气道重塑的确切机制及其功能后果。
