Biswas K, Chang A, Hoggard M, Radcliff F J, Jiang Y, Taylor M W, Darveau R, Douglas R G
Department of Surgery, University of Auckland, New Zealand.
School of Dentistry, University of Washington, Seattle, WA, USA.
Rhinology. 2017 Mar 1;55(1):59-69. doi: 10.4193/Rhino16.201.
The sino-nasal disease chronic rhinosinusitis (CRS) is primarily an inflammatory condition that manifests in several ways. However, the aetiology of this complex disease is poorly understood. The aim of this study was to explore the association between toll-like receptor (TLR) activation, host immune response and sino-nasal mucus in healthy and diseased patients.
The activation of TLR2/1 and TLR4 by sino-nasal mucus from 26 CRS patients and 10 healthy controls was measured. In addition, 7 inflammatory cytokines, bacterial community composition and bacterial abundance within the sino-nasal mucus were measured using molecular and diagnostic tools.
TLR activity was observed in 9/36 samples, including 2 healthy controls. There was a strong, positive correlation between members of the Gammaproteobacteria (Haemophilus, Enterobacter, Pseudomonas) and TLR2/1 and TLR4 activity. Bacterial abundance and cytokine (tumour necrosis factor) abundance were also positively correlated with TLR activity.
These findings suggest that a small proportion (20-30%) of individuals in each sub-group are more predisposed to TLR activity, which may be related to bacterial composition, diversity and abundance in the sinuses.
鼻鼻窦疾病慢性鼻-鼻窦炎（CRS）主要是一种以多种方式表现出来的炎症性疾病。然而，这种复杂疾病的病因尚不清楚。本研究的目的是探讨健康和患病患者中 Toll 样受体（TLR）激活、宿主免疫反应与鼻鼻窦黏液之间的关联。
检测了 26 例 CRS 患者和 10 例健康对照的鼻鼻窦黏液对 TLR2/1 和 TLR4 的激活情况。此外，使用分子和诊断工具检测了鼻鼻窦黏液中的 7 种炎性细胞因子、细菌群落组成和细菌丰度。
在 36 个样本中的 9 个样本中观察到 TLR 活性，其中包括 2 例健康对照。γ-变形菌纲（嗜血杆菌属、肠杆菌属、假单胞菌属）成员与 TLR2/1 和 TLR4 活性之间存在强正相关。细菌丰度和细胞因子（肿瘤坏死因子）丰度也与 TLR 活性呈正相关。
这些发现表明，每个亚组中一小部分（20%-30%）个体更易发生 TLR 活性，这可能与鼻窦中的细菌组成、多样性和丰度有关。
