单端孢霉烯族毒素在拟分枝孢镰刀菌NRRL 3299突变体的液体培养物中积累。
Trichothecenes accumulated in liquid culture of a mutant of Fusarium sporotrichioides NRRL 3299.
作者信息
Plattner R D, Tjarks L W, Beremand M N
机构信息
Northern Regional Research Center, U.S. Department of Agriculture, Peoria, Illinois 61604.
出版信息
Appl Environ Microbiol. 1989 Sep;55(9):2190-4. doi: 10.1128/aem.55.9.2190-2194.1989.
Abstract
A UV-generated mutant of Fusarium sporotrichioides NRRL 3299 was altered in its ability to biosynthesize T-2 toxin, as shown by a rapid screen with monoclonal antibodies to T-2. This stable mutant accumulated two trichothecenes that were not observed in liquid cultures of the parent strain. The two compounds were identified as 3,15-diol 12,13-epoxytrichothec-9-ene and 3,15-diol 12,13-epoxytrichothec-9-ene 3-acetate on the basis of their nuclear magnetic resonance and mass spectra. This is the first report of either of these two compounds as secondary metabolites of F. sporotrichioides and of a trichothecene acetylated at C-3 by this species.
摘要
如用针对T-2的单克隆抗体进行快速筛选所示，紫外线诱导的禾谷镰刀菌NRRL 3299突变体在生物合成T-2毒素的能力方面发生了改变。这个稳定的突变体积累了两种在亲本菌株液体培养物中未观察到的单端孢霉烯族毒素。根据它们的核磁共振和质谱，这两种化合物被鉴定为3,15 -二醇12,13 -环氧单端孢霉-9 -烯和3,15 -二醇12,13 -环氧单端孢霉-9 -烯3 -乙酸酯。这是关于这两种化合物作为禾谷镰刀菌次生代谢产物以及该物种在C-3位乙酰化的单端孢霉烯族毒素的首次报道。
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