木霉梭孢镰孢菌中 trichothecene 的生物合成：T-2 毒素氧原子的起源。
Trichothecene Biosynthesis in Fusarium sporotrichioides: Origin of the Oxygen Atoms of T-2 Toxin.
机构信息
Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604.
出版信息
Appl Environ Microbiol. 1986 Mar;51(3):493-7. doi: 10.1128/aem.51.3.493-497.1986.
Abstract
Mass spectral analysis of T-2 toxin formed during the growth of Fusarium sporotrichioides (ATCC 24043) in the presence of H(2)O showed incorporation of up to three O atoms per toxin molecule. The carbonyl oxygens of the acetates at C-4 and C-15 and of the isovalerate at C-8 were derived from H(2)O. Toxin formed in the presence of O molecular oxygen incorporated up to six O atoms per toxin molecule. The overall incorporation was 78 and 92% of toxin molecules labeled for H(2)O and O(2) labeled samples, respectively. The oxygens of position 1, the 12,13-epoxide, and the hydroxyl groups at C-3, C-4, C-8, and C-15 were all derived from molecular oxygen.
摘要
在存在 H（2）O 的情况下，对在 Fusarium sporotrichioides（ATCC 24043）生长过程中形成的 T-2 毒素进行质谱分析表明，每个毒素分子最多可掺入三个氧原子。C-4 和 C-15 位的乙酸盐以及 C-8 位的异戊酸盐的羰基氧来自 H（2）O。在存在 O 分子氧的情况下形成的毒素，每个毒素分子最多可掺入六个氧原子。分别用 H（2）O 和 O（2）标记的样品中，毒素分子的总掺入率分别为 78%和 92%。位置 1、12,13-环氧化物以及 C-3、C-4、C-8 和 C-15 位的羟基的氧均来自分子氧。
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