拟枝孢镰刀菌NRRL 3299突变菌株对可能的T-2毒素前体的生物转化
Bioconversion of possible T-2 toxin precursors by a mutant strain of Fusarium sporotrichioides NRRL 3299.
作者信息
McCormick S P, Taylor S L, Plattner R D, Beremand M N
机构信息
Northern Regional Research Center, U.S. Department of Agriculture, Peoria, Illinois 61604.
出版信息
Appl Environ Microbiol. 1990 Mar;56(3):702-6. doi: 10.1128/aem.56.3.702-706.1990.
Abstract
Liquid cultures of a mutant strain of Fusarium sporotrichioides NRRL 3299 that accumulates trichodiene rather than T-2 toxin converted tricho-9-ene-2 alpha,3 alpha,11 alpha-triol, trichotriol (tricho-10-ene-2 alpha,3 alpha,9 alpha-triol), tricho-10-ene-2 alpha,3 alpha,9 beta-triol, 3 alpha-hydroxytrichothecene, and 3 alpha-acetoxytrichothecene to T-2 toxin. Other possible oxygenated precursors of T-2 toxin, including trichodiol (tricho-10-ene-2 alpha,9 alpha-diol), trichothecene, 4 alpha-hydroxytrichothecene, and 15-hydroxytrichothecene, were not metabolized. The results indicate that in the biosynthesis of T-2 toxin by F. sporotrichioides, (i) oxygenation at C-3 occurs prior to the second cyclization, (ii) this second cyclization involves two steps that may be nonenzymatic, and (iii) oxidation at C-3 precedes that at C-4 or C-15.
摘要
串珠镰刀菌突变株NRRL 3299的液体培养物积累了单端孢霉烯而不是T-2毒素，该培养物将单端孢霉-9-烯-2α,3α,11α-三醇、单端孢三醇（单端孢-10-烯-2α,3α,9α-三醇）、单端孢-10-烯-2α,3α,9β-三醇、3α-羟基单端孢霉烯和3α-乙酰氧基单端孢霉烯转化为T-2毒素。T-2毒素的其他可能的氧化前体，包括单端孢二醇（单端孢-10-烯-2α,9α-二醇）、单端孢霉烯、4α-羟基单端孢霉烯和15-羟基单端孢霉烯，未被代谢。结果表明，在串珠镰刀菌生物合成T-2毒素的过程中，（i）C-3位的氧化发生在第二次环化之前，（ii）第二次环化涉及两个可能是非酶促的步骤，（iii）C-3位的氧化先于C-4或C-15位的氧化。
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本文引用的文献
1
Trichothecene Biosynthesis in Fusarium sporotrichioides: Origin of the Oxygen Atoms of T-2 Toxin.木霉梭孢镰孢菌中 trichothecene 的生物合成：T-2 毒素氧原子的起源。
Appl Environ Microbiol. 1986 Mar;51(3):493-7. doi: 10.1128/aem.51.3.493-497.1986.
2
Identification of averantin as an aflatoxin B1 precursor: placement in the biosynthetic pathway.鉴定averantin为黄曲霉毒素B1前体：确定其在生物合成途径中的位置。
Appl Environ Microbiol. 1980 Apr;39(4):835-9. doi: 10.1128/aem.39.4.835-839.1980.
3
Trichothecenes, 1: The synthesis of 4-deoxyverrucarol from verrucarol and diacetoxyscirpenol.
J Nat Prod. 1984 May-Jun;47(3):514-9. doi: 10.1021/np50033a019.
4
Detection and analysis of epoxides with 4-(p-Nitrobenzyl)-pyridine.用4-（对硝基苄基）吡啶检测和分析环氧化物
Bull Environ Contam Toxicol. 1974 Dec;12(6):759-64. doi: 10.1007/BF01685927.
5
Purification and characterization of the sesquiterpene cyclase trichodiene synthetase from Fusarium sporotrichioides.来自拟分枝孢镰刀菌的倍半萜环化酶 trichodiene 合成酶的纯化与表征。
Arch Biochem Biophys. 1986 Dec;251(2):756-61. doi: 10.1016/0003-9861(86)90386-3.
6
Isolation and characterization of mutants blocked in T-2 toxin biosynthesis.T-2毒素生物合成受阻突变体的分离与鉴定
Appl Environ Microbiol. 1987 Aug;53(8):1855-9. doi: 10.1128/aem.53.8.1855-1859.1987.
7
Averufanin is an aflatoxin B1 precursor between averantin and averufin in the biosynthetic pathway.阿弗菌素是生物合成途径中阿弗拉菌素和阿弗菌素之间的黄曲霉毒素B1前体。
Appl Environ Microbiol. 1987 Jan;53(1):14-6. doi: 10.1128/aem.53.1.14-16.1987.
8
New modified trichothecenes accumulated in solid culture by mutant strains of Fusarium sporotrichioides.新的修饰型单端孢霉烯族毒素由拟分枝孢镰刀菌突变菌株在固体培养中积累产生。
Appl Environ Microbiol. 1989 Sep;55(9):2195-9. doi: 10.1128/aem.55.9.2195-2199.1989.
9
Trichothecenes accumulated in liquid culture of a mutant of Fusarium sporotrichioides NRRL 3299.单端孢霉烯族毒素在拟分枝孢镰刀菌NRRL 3299突变体的液体培养物中积累。
Appl Environ Microbiol. 1989 Sep;55(9):2190-4. doi: 10.1128/aem.55.9.2190-2194.1989.
10
Isolation and nucleotide sequence of a sesquiterpene cyclase gene from the trichothecene-producing fungus Fusarium sporotrichioides.从产单端孢霉烯族毒素的真菌拟分枝孢镰刀菌中分离出倍半萜环化酶基因及其核苷酸序列
Gene. 1989 Jun 30;79(1):131-8. doi: 10.1016/0378-1119(89)90098-x.
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