McCormick S P, Taylor S L, Plattner R D, Beremand M N
Northern Regional Research Center, U.S. Department of Agriculture, Peoria, Illinois 61604.
Appl Environ Microbiol. 1990 Mar;56(3):702-6. doi: 10.1128/aem.56.3.702-706.1990.
Liquid cultures of a mutant strain of Fusarium sporotrichioides NRRL 3299 that accumulates trichodiene rather than T-2 toxin converted tricho-9-ene-2 alpha,3 alpha,11 alpha-triol, trichotriol (tricho-10-ene-2 alpha,3 alpha,9 alpha-triol), tricho-10-ene-2 alpha,3 alpha,9 beta-triol, 3 alpha-hydroxytrichothecene, and 3 alpha-acetoxytrichothecene to T-2 toxin. Other possible oxygenated precursors of T-2 toxin, including trichodiol (tricho-10-ene-2 alpha,9 alpha-diol), trichothecene, 4 alpha-hydroxytrichothecene, and 15-hydroxytrichothecene, were not metabolized. The results indicate that in the biosynthesis of T-2 toxin by F. sporotrichioides, (i) oxygenation at C-3 occurs prior to the second cyclization, (ii) this second cyclization involves two steps that may be nonenzymatic, and (iii) oxidation at C-3 precedes that at C-4 or C-15.
串珠镰刀菌突变株NRRL 3299的液体培养物积累了单端孢霉烯而不是T-2毒素，该培养物将单端孢霉-9-烯-2α,3α,11α-三醇、单端孢三醇（单端孢-10-烯-2α,3α,9α-三醇）、单端孢-10-烯-2α,3α,9β-三醇、3α-羟基单端孢霉烯和3α-乙酰氧基单端孢霉烯转化为T-2毒素。T-2毒素的其他可能的氧化前体，包括单端孢二醇（单端孢-10-烯-2α,9α-二醇）、单端孢霉烯、4α-羟基单端孢霉烯和15-羟基单端孢霉烯，未被代谢。结果表明，在串珠镰刀菌生物合成T-2毒素的过程中，（i）C-3位的氧化发生在第二次环化之前，（ii）第二次环化涉及两个可能是非酶促的步骤，（iii）C-3位的氧化先于C-4或C-15位的氧化。
