School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center of Sensor Materials and Applications, Zhejiang University , Hangzhou 310027, China.
ACS Appl Mater Interfaces. 2017 Jan 25;9(3):2130-2137. doi: 10.1021/acsami.6b14182. Epub 2017 Jan 10.
Light-induced cell detachment shows much potential in in vitro cell culture and calls for high-performance light-responsive films. In this study, a smooth and dense SiO/TiO nanocomposite thin film with thickness of around 250 nm was first fabricated on HO treated polystyrene (PS) substrate via a low-temperature sol-gel method. It was observed that the film could well-adhere on the PS surface and the bonding strength became increasingly high with the increase of SiO content. The peeling strength and shear strength reached 3.05 and 30.02 MPa, respectively. It was observed the surface of the film could transform into superhydrophilic upon 20 min illumination of ultraviolet with a wavelength of 365 nm (UV365). In cell culture, cells, i.e., NIH3T3 and MC3T3-E1 cells, cultured on SiO/TiO nanocomposite film were easily detached after 10 min of UV365 illumination; the detachment rates reached 90.8% and 88.6%, respectively. Correspondingly, continuous cell sheets with good viability were also easily obtained through the same way. The present work shows that SiO/TiO nanocomposite thin film could be easily prepared on polymeric surface at low temperature. The corresponding film exhibits excellent biocompatibility, high bonding strength, and good light responses. It could be a good candidate for the surface of cell culture utensils with light-induced cell detachment property.
光照诱导细胞脱落在体外细胞培养中具有很大的潜力，因此需要高性能的光响应薄膜。在本研究中，通过低温溶胶-凝胶法在 HO 处理的聚苯乙烯（PS）基底上首先制备了厚度约为 250nm 的光滑致密的 SiO/TiO 纳米复合薄膜。结果表明，薄膜能够很好地附着在 PS 表面上，并且随着 SiO 含量的增加，键合强度逐渐增加。剥离强度和剪切强度分别达到 3.05MPa 和 30.02MPa。当用波长为 365nm 的紫外光（UV365）照射 20min 时，观察到薄膜表面可以转变为超亲水状态。在细胞培养中，培养在 SiO/TiO 纳米复合薄膜上的 NIH3T3 和 MC3T3-E1 细胞在经过 10min 的 UV365 光照后很容易脱落，脱落率分别达到 90.8%和 88.6%。相应地，通过同样的方法也很容易获得具有良好活力的连续细胞片。本工作表明，SiO/TiO 纳米复合薄膜可以在低温下很容易地在聚合物表面上制备。相应的薄膜具有优异的生物相容性、高键合强度和良好的光响应性能。它可能是具有光诱导细胞脱落性能的细胞培养器具表面的良好候选材料。
