Long Xiaojun, Yi Yang, Wang Xiaozhao, Duan Xiyue, Sun Yuan, Wu Chengwei, Weng Wenjian, Xu Boyong, Cheng Kui, Wang Huiming, Lin Jun
School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China.
Zhejiang University-University of Edinburgh Institute and School of Basic Medicine, Zhejiang University School of Medicine, Hangzhou 314400, China.
ACS Biomater Sci Eng. 2020 Apr 13;6(4):2020-2028. doi: 10.1021/acsbiomaterials.9b01946. Epub 2020 Mar 9.
Light-induced cell harvest shows much potential in in vitro cell culture. In this work, a light-responsive monolayer graphene (Gr)/titanium dioxide nanodot (TN) film is designed and used for light-induced cell harvest. It is found that after 20 min of 365 nm UV or 450 nm visible light illumination, different types of cells could be detached from the surface effectively. The highest cell detachment ratio reaches about 95%. The mechanism of such a cell detachment is contributed to light illumination generates charge accumulation, which, in turn, changes the conformation of the extracellular matrix protein molecules adsorbed to a more disordered state, and eventually leads to the cells detachment. Such UV and visible light responsive Gr/TiO film could be a good candidate for a surface with light-induced cell detachment property.
光诱导细胞收获在体外细胞培养中显示出很大潜力。在这项工作中，设计并使用了一种光响应单层石墨烯（Gr）/二氧化钛纳米点（TN）薄膜用于光诱导细胞收获。研究发现，在365 nm紫外线或450 nm可见光照射20分钟后，不同类型的细胞能够有效地从表面脱离。最高细胞脱离率达到约95%。这种细胞脱离的机制是光照产生电荷积累，进而使吸附的细胞外基质蛋白分子构象改变为更无序的状态，最终导致细胞脱离。这种对紫外线和可见光响应的Gr/TiO薄膜可能是具有光诱导细胞脱离特性表面的良好候选材料。
