Jiang Zhiwei, Li Na, Shao Qin, Zhu Danji, Feng Yuting, Wang Yang, Yu Mengjia, Ren Lingfei, Chen Qianming, Yang Guoli
Stomatology Hospital, School of Stomatology Zhejiang University School of Medicine Hangzhou Zhejiang China.
Zhejiang Provincial Clinical Research Center for Oral Diseases Hangzhou Zhejiang China.
Bioeng Transl Med. 2022 May 13;8(1):e10334. doi: 10.1002/btm2.10334. eCollection 2023 Jan.
Cell aggregates that mimic in vivo cell-cell interactions are promising and powerful tools for tissue engineering. This study isolated a new, easily obtained, population of mesenchymal stem cells (MSCs) from rat hard palates named hard palatal-derived mesenchymal stem cells (PMSCs). The PMSCs were positive for CD90, CD44, and CD29 and negative for CD34, CD45, and CD146. They exhibited clonogenicity, self-renewal, migration, and multipotent differentiation capacities. Furthermore, this study fabricated scaffold-free 3D aggregates using light-controlled cell sheet technology and a serum-free method. PMSC aggregates were successfully constructed with good viability. Transplantation of the PMSC aggregates and the PMSC aggregate-implant complexes significantly enhanced bone formation and implant osseointegration in vivo, respectively. This new cell resource is easy to obtain and provides an alternative strategy for tissue engineering and regenerative medicine.
模拟体内细胞间相互作用的细胞聚集体是组织工程中很有前景且强大的工具。本研究从大鼠硬腭分离出一种新的、易于获取的间充质干细胞（MSC）群体，命名为硬腭来源的间充质干细胞（PMSC）。PMSC对CD90、CD44和CD29呈阳性，对CD34、CD45和CD146呈阴性。它们表现出克隆形成能力、自我更新能力、迁移能力和多能分化能力。此外，本研究使用光控细胞片技术和无血清方法制备了无支架三维聚集体。成功构建了具有良好活力的PMSC聚集体。PMSC聚集体和PMSC聚集体 - 植入物复合物的移植分别在体内显著增强了骨形成和植入物骨整合。这种新的细胞资源易于获取，为组织工程和再生医学提供了一种替代策略。
