Xu Jufei, Liu Xiangzhe, Liang Pengpeng, Yuan Hailong, Yang Tianyou
Department of Pharmacy, Air Force Medical Center, PLA, Air Force Medical University, Beijing 100142, China.
School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
Pharmaceutics. 2024 Apr 13;16(4):538. doi: 10.3390/pharmaceutics16040538.
The improvement of the capability of poly(-isopropylacrylamide) (PNIPAAm) hydrogel coating in cell adhesion and detachment is critical to efficiently prepare cell sheets applied in cellular therapies and tissue engineering. To enhance cell response on the surface, the amine group-modified PNIPAAm (PNIPAAm-APTES) nanohydrogels were synthesized and deposited spontaneously on tannic acid (TA)-modified polyethylene (PE) plates. Subsequently, TA was introduced onto PNIPAAm-APTES nanohydrogels to fabricate coatings composed of TA-modified PNIPAAm-APTES (PNIPAAm-APTES-TA). Characterization techniques, including TEM, SEM, XPS, and UV-Vis spectroscopy, confirmed the effective deposition of hydrogels of PNIPAAm as well as the morphologies, content of chemical bonding-TA, and stability of various coatings. Importantly, the porous hydrogel coatings exhibited superhydrophilicity at 20 °C and thermo-responsive behavior. The fluorescence measurement demonstrated that the coating's stability effectively regulated protein behavior, influencing cell response. Notably, cell response tests revealed that even without precise control over the chain length/thickness of PNIPAAm during synthesis, the coatings enhanced cell adhesion and detachment, facilitating efficient cell culture. This work represented a novel and facile approach to preparing bioactive PNIPAAm for cell culture.
聚（N-异丙基丙烯酰胺）（PNIPAAm）水凝胶涂层在细胞黏附与脱离能力方面的改进对于高效制备用于细胞治疗和组织工程的细胞片至关重要。为了增强表面的细胞反应，合成了胺基修饰的PNIPAAm（PNIPAAm-APTES）纳米水凝胶，并将其自发沉积在单宁酸（TA）修饰的聚乙烯（PE）板上。随后，将TA引入PNIPAAm-APTES纳米水凝胶中，以制备由TA修饰的PNIPAAm-APTES（PNIPAAm-APTES-TA）组成的涂层。包括透射电子显微镜（TEM）、扫描电子显微镜（SEM）、X射线光电子能谱（XPS）和紫外可见光谱在内的表征技术证实了PNIPAAm水凝胶的有效沉积以及各种涂层的形态、化学键合TA的含量和稳定性。重要的是，多孔水凝胶涂层在20℃时表现出超亲水性和热响应行为。荧光测量表明，涂层的稳定性有效地调节了蛋白质行为，影响了细胞反应。值得注意的是，细胞反应测试表明，即使在合成过程中没有精确控制PNIPAAm的链长/厚度，这些涂层也增强了细胞黏附与脱离，促进了高效细胞培养。这项工作代表了一种制备用于细胞培养的生物活性PNIPAAm的新颖且简便的方法。
