Sun Xiaoxue, Zhang Jingyu, Han Xiaohui, Li Shumin, Zhang Xuerui, Bi Xiaodong
School of Pharmaceutical Sciences & Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences Jinan 250117 Shandong China
Key Laboratory for Biotechnology Drugs of National Health Commission (Shandong Academy of Medical Sciences) Jinan 250117 Shandong China.
RSC Adv. 2024 Apr 30;14(20):14202-14213. doi: 10.1039/d3ra08576g. eCollection 2024 Apr 25.
In the search for pharmaceutically active compounds from natural products, it is crucial and challenging to develop separation methods that target not only structurally similar compounds but also a class of compounds with desired pharmaceutical functions. To achieve both structure-oriented and function-oriented selectivity, the choice of functional monomers with broad interactions or even biomimetic roles towards targeted compounds is essential. In this work, an imidazole (IM)-functionalized paper membrane was synthesized to realize selectivity. The IM was selected based on its capability to provide multiple interactions, participation in several bioprocesses, and experimental verification of adsorption performance. Using gallic acid as a representative component of , the preparation conditions and extraction parameters were systematically investigated. The optimal membrane solid-phase extraction (MSPE) method allowed for enrichment of gallic acid from the complex matrix of , enabling facile quantitative analysis with a limit of detection (LOD) of 0.1 ng mL. Furthermore, with the aid of cheminformatics, the extracted compounds were found to be similar in both their structures and pharmaceutical functions. This work offers a novel approach to preparing a readily synthesized extraction membrane capable of isolating compounds with similar structures and pharmaceutical effects, and provides an MSPE-based analytical method for natural products.
在从天然产物中寻找具有药物活性的化合物时，开发不仅针对结构相似化合物，而且针对一类具有所需药物功能的化合物的分离方法至关重要且具有挑战性。为了实现结构导向和功能导向的选择性，选择对目标化合物具有广泛相互作用甚至仿生作用的功能单体至关重要。在这项工作中，合成了一种咪唑（IM）功能化的纸膜以实现选择性。选择IM是基于其提供多种相互作用、参与多种生物过程以及吸附性能的实验验证。以没食子酸作为 的代表性成分，系统研究了制备条件和萃取参数。优化的膜固相萃取（MSPE）方法能够从 的复杂基质中富集没食子酸，实现简便的定量分析，检测限（LOD）为0.1 ng/mL。此外，借助化学信息学，发现提取的化合物在结构和药物功能上都相似。这项工作提供了一种制备易于合成的萃取膜的新方法，该膜能够分离具有相似结构和药物作用的化合物，并为天然产物提供了一种基于MSPE的分析方法。
