Ansari Muhammad Ridwan, Agustina Rina, Khusun Helda, Prafiantini Erfi, Cahyaningrum Fitrianna, Permadhi Inge
Department of Nutrition, Faculty of Medicine, Universitas Indonesia - Dr Cipto Mangunkusumo General Hospital Jakarta, Indonesia.
Southeast Asian Ministers of Education Organization Regional Centre for Food and Nutrition (SEAMEO RECFON)/ Pusat Kajian Gizi Regional (PKGR) Universitas Indonesia, Jakarta, Indonesia.
Asia Pac J Clin Nutr. 2016 Dec;25(Suppl 1):S20-S29. doi: 10.6133/apjcn.122016.s4.
A balance ratio of dietary omega-3 (n-3) and omega-6 (n-6) fatty acids reduces childhood obesity. However, few studies have focused on validation of semiquantitative food frequency questionnaire (SFFQ) for determining the n-3 and n-6 intakes in children. Therefore, a valid SFFQ for assessing n-3 and n-6 intakes among Indonesian children is required.
A cross-sectional study was conducted by selecting 89 healthy children through multistage random sampling. Dietary intakes were assessed using the SFFQ and a 3-day non-consecutive 24-h recall. Randomly selected children (n=35) were assessed for plasma phospholipid fatty acid (PFA). In total, 78 food items in the SFFQ, as in the Thai, Vietnamese, and American food composition databases, were validated using dietary recall and PFA. The SFFQ was readministered after 4 weeks to assess its reproducibility. The validity and reproducibility of the SFFQ were determined by Bland-Altman analysis.
Favourable agreement was found between the SFFQ and recall for docosahexanoic acid, eicosapentanoic acid, docosapentanoic acid, and arachidonic acid, but not for total n-3, n-6, α-linolenic acid, or linoleic acid. Significant correlations were found between the SFFQ estimations and plasma n-6 and LA (r=0.40, p=0.025; and r=0.42, p=0.018, respectively). A 95% limit of Bland-Altman agreement was observed between the first and repeat SFFQ for all fatty acids.
The proposed SFFQ is sufficiently valid and reliable for assessment of essential fatty acids intakes in Indonesian children.
膳食中ω-3（n-3）和ω-6（n-6）脂肪酸的平衡比例可降低儿童肥胖率。然而，很少有研究关注用于确定儿童n-3和n-6摄入量的半定量食物频率问卷（SFFQ）的验证。因此，需要一种有效的SFFQ来评估印度尼西亚儿童的n-3和n-6摄入量。
通过多阶段随机抽样选择89名健康儿童进行横断面研究。使用SFFQ和3天非连续24小时回顾法评估膳食摄入量。随机选择的儿童（n = 35）进行血浆磷脂脂肪酸（PFA）评估。SFFQ中的78种食物，如同泰国、越南和美国食物成分数据库中的一样，使用膳食回顾法和PFA进行验证。4周后再次发放SFFQ以评估其可重复性。通过Bland-Altman分析确定SFFQ的有效性和可重复性。
在二十二碳六烯酸、二十碳五烯酸、二十二碳五烯酸和花生四烯酸方面，SFFQ与回顾法之间达成了良好的一致性，但在总n-3、n-6、α-亚麻酸或亚油酸方面则不然。SFFQ估计值与血浆n-6和亚油酸之间存在显著相关性（分别为r = 0.40，p = 0.025；r = 0.42，p = 0.018）。所有脂肪酸的首次和重复SFFQ之间观察到Bland-Altman一致性的95%界限。
所提出的SFFQ对于评估印度尼西亚儿童必需脂肪酸摄入量具有足够的有效性和可靠性。
