Orton H D, Szabo N J, Clare-Salzler M, Norris J M
Department of Preventive Medicine and Biometrics, University of Colorado at Denver and Health Sciences Center, Denver, CO 80262, USA.
Eur J Clin Nutr. 2008 Jun;62(6):733-8. doi: 10.1038/sj.ejcn.1602763. Epub 2007 Apr 18.
We conducted a dietary validation study in youth aged 1-11 years by comparing dietary intake of omega-3 and omega-6 polyunsaturated fatty acids (PUFAs) as assessed by a parent-completed semiquantitative food frequency questionnaire (FFQ) over time to erythrocyte membrane composition of the same fatty acids.
The study population included youth aged 1-11 years who were participants in the Diabetes Autoimmunity Study in the Young (DAISY), a longitudinal study in Denver, Colorado that is following a cohort of youth at risk for developing type I diabetes. Four hundred and four children who had erythrocyte membrane fatty acid data matched to an FFQ corresponding to the same time frame for a total of 917 visits (matches) were included. PUFA intake was expressed both as g/day (adjusted for total energy) and as percent of total fat intake. We used mixed models to test the association and calculate the correlation between the erythrocyte membrane estimates and PUFA intake using all records of data for each youth.
Intakes of total omega-3 fatty acids (beta=0.52, P<0.0001, rho=0.23) and marine PUFAs (beta=1.62, P<0.0001, rho=0.42), as a percent of total fat in the diet, were associated with percent of omega-3 and marine PUFAs in the erythrocyte membrane. Intakes of omega-6 PUFAs (beta=0.04, P=0.418, rho=0.05) and arachidonic acid (beta=0.31, P=0.774, rho=0.01) were not associated.
In these young children, an FFQ using parental report provided estimates of average long-term intakes of marine PUFAs that correlated well with their erythrocyte cell membrane fatty acid status.
我们通过比较由家长完成的半定量食物频率问卷（FFQ）评估的1-11岁青少年随时间变化的ω-3和ω-6多不饱和脂肪酸（PUFA）的饮食摄入量与相同脂肪酸的红细胞膜组成，进行了一项饮食验证研究。
研究人群包括1-11岁的青少年，他们是青少年糖尿病自身免疫研究（DAISY）的参与者，这是一项在科罗拉多州丹佛市进行的纵向研究，跟踪一组有患I型糖尿病风险的青少年。共有917次就诊（匹配），纳入了404名有与同一时间框架对应的FFQ匹配的红细胞膜脂肪酸数据的儿童。PUFA摄入量既以克/天（根据总能量调整）表示，也以总脂肪摄入量的百分比表示。我们使用混合模型来检验这种关联，并使用每个青少年的所有数据记录计算红细胞膜估计值与PUFA摄入量之间的相关性。
饮食中总ω-3脂肪酸（β=0.52，P<0.0001，ρ=0.23）和海洋PUFA（β=1.62，P<0.0001，ρ=0.42）占总脂肪的百分比与红细胞膜中ω-3和海洋PUFA的百分比相关。ω-6 PUFA（β=0.04，P=0.418，ρ=0.05）和花生四烯酸（β=0.31，P=0.774，ρ=0.01）的摄入量无相关性。
在这些幼儿中，使用家长报告的FFQ提供的海洋PUFA平均长期摄入量估计值与他们的红细胞膜脂肪酸状态相关性良好。
