Yang Qingpo, Li Zhen, Li Hui, Li Yanrong, Yang Yuhui, Zhang Qingwen, Liu Xiaoxia
Department of Entomology, China Agricultural University, Beijing 100193, China (
Environ Entomol. 2016 Dec;45(6):1552-1560. doi: 10.1093/ee/nvw119. Epub 2016 Sep 11.
After injury many insects could regenerate lost limb. In this study, Helicoverpa armigera Hubner and Locusta migratoria manilensis (Meyen, 1835) were chosen to compare the regeneration potency of holometabolous and hemimetabolous insects. We employed the classical approach of surgical excision to verify the regeneration ability and to investigate the factors that affect the extent of regeneration. The results found that H. armigera could regenerate intact legs when the larval legs were excised at the first and second instar and that legs of adult H. armigera had a close relationship with their larval counterparts. However, the adult legs became malformed or disappeared when excised at other older instars. For the L. migratoria, we found the legs have weak partial regeneration ability when amputation was conducted at the joint of two segments. The regeneration potency might be stronger the more proximal the operation. Regeneration process had a negative impact on the larval development. This is the first report of complete leg regeneration capacity having a strong correlation with the instar but not with the position where amputation occurred for H. armigera, while for the L. migratoria, partial regenerative ability had a close relationship with the position where amputation occurred but not with instars.
受伤后，许多昆虫能够再生失去的肢体。在本研究中，选择了棉铃虫和东亚飞蝗来比较全变态昆虫和不完全变态昆虫的再生能力。我们采用经典的手术切除方法来验证再生能力，并研究影响再生程度的因素。结果发现，当棉铃虫幼虫在一龄和二龄时切除腿部，能够再生出完整的腿部，并且棉铃虫成虫的腿部与其幼虫时期的腿部密切相关。然而，在其他大龄幼虫期切除腿部时，成虫的腿部会出现畸形或消失。对于东亚飞蝗，我们发现当在两个体节的关节处进行截肢时，腿部具有较弱的部分再生能力。手术部位越靠近身体近端，再生能力可能越强。再生过程对幼虫发育有负面影响。这是首次报道，对于棉铃虫，完整腿部的再生能力与龄期密切相关，而与截肢位置无关；而对于东亚飞蝗，部分再生能力与截肢位置密切相关，与龄期无关。
