Arbefeville Sophie, Ferrieri Patricia
From the Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis.
Am J Clin Pathol. 2017 Jan 1;147(1):43-49. doi: 10.1093/ajcp/aqw185.
To investigate the etiology of viral respiratory tract infections mainly in hospitalized children and adults over a 12-month consecutive period after implementation of a 14-virus multiplex nucleic acid amplification test.
From January 2014 to January 2015, a total of 2,237 respiratory samples were analyzed with the US Food and Drug Administration-cleared eSensor Respiratory Viral Panel (GenMark Diagnostics, Carlsbad, CA).
Of the 2,237 specimens tested, 788 specimens were positive for at least one virus, giving a positivity rate of 35.2%, and because of viral codetection, a total of 862 viral targets were identified. The age groups with the highest positivity rates were the 0- to 1-year (73.5%) and 2- to 6-year (78.4%) age groups. The overall viral codetection rate was 9.1%. Human rhinovirus (HRV) was the most prevalent respiratory virus found in children and adults. The peak of HRV seen in September 2014 represented a combination of HRV and enterovirus D68, 2014 epidemic respiratory infections.
The ability to detect a wider range of respiratory viruses gave us a better understanding of the etiology of respiratory infections in our population, particularly for HRV and enhanced our ability to detect viral coinfection.
在实施一项可检测14种病毒的多重核酸扩增检测之后，连续12个月调查以住院儿童和成人为主要对象的病毒性呼吸道感染的病因。
2014年1月至2015年1月，使用经美国食品药品监督管理局批准的eSensor呼吸道病毒检测板（GenMark Diagnostics公司，加利福尼亚州卡尔斯巴德）对总共2237份呼吸道样本进行了分析。
在检测的2237份标本中，788份标本至少对一种病毒呈阳性，阳性率为35.2%，由于病毒共检测，总共鉴定出862个病毒靶点。阳性率最高的年龄组是0至1岁（73.5%）和2至6岁（78.4%）年龄组。总体病毒共检测率为9.1%。人鼻病毒（HRV）是在儿童和成人中发现的最常见的呼吸道病毒。2014年9月出现的HRV高峰代表了HRV与肠道病毒D68的合并感染，即2014年流行的呼吸道感染。
检测更广泛的呼吸道病毒的能力使我们更好地了解了我们人群中呼吸道感染的病因，特别是对于HRV，并增强了我们检测病毒合并感染的能力。
