Amr Khalda, El-Awady Rehab, Raslan Hala
National Research Center, Internal Medicine, Cairo, Egypt.
Al-Azhar University Faculty of Pharmacy, Biochemistry, Cairo, Egypt.
Open Access Maced J Med Sci. 2016 Dec 15;4(4):574-577. doi: 10.3889/oamjms.2016.110. Epub 2016 Oct 11.
This study aimed to investigate genotype and allele frequencies of -174 (rs1800795) and (rs1800796) promoter gene polymorphisms in Egyptian patients with rheumatoid arthritis (RA) in comparison to control group.
The study was conducted on 198 Egyptian subjects (99 RA patients and 99 healthy control). The promoter region of gene was amplified by PCR using DNAs from patients and the controls, and their PCR products were digested by suitable enzymes.
No statistical differences were found in genotype (P = 0.177) or allele (P = 0.147) frequencies between RA patients and controls. Significant differences were observed in genotype (P < 0.001) and allele (P < 0.001) frequencies between RA patients and controls.
A significant association of gene polymorphism and RA in Egyptian population was found with significantly higher frequencies of and genotypes and allele in RA patients compared to controls. No association was found between gene polymorphism and RA.
本研究旨在调查埃及类风湿关节炎（RA）患者与对照组相比，-174（rs1800795）和（rs1800796）启动子基因多态性的基因型和等位基因频率。
该研究对198名埃及受试者（99例RA患者和99名健康对照）进行。使用患者和对照的DNA通过PCR扩增基因的启动子区域，并用合适的酶消化其PCR产物。
RA患者与对照组之间的基因型（P = 0.177）或等位基因（P = 0.147）频率未发现统计学差异。RA患者与对照组之间的基因型（P < 0.001）和等位基因（P < 0.001）频率存在显著差异。
在埃及人群中发现基因多态性与RA存在显著关联，与对照组相比，RA患者中基因型和等位基因的频率显著更高。未发现基因多态性与RA之间存在关联。
