白细胞介素-6基因多态性的174 G/C和-572 G/C与伊朗人群乳糜泻易感性之间的关系。
The relationship between 174 G/C and -572 G/C of IL-6 gene polymorphisms and susceptibility of celiac disease in the Iranian population.
作者信息
Barartabar Zeinab, Nikzamir Abdolrahim, Sirati-Sabet Majid, Aghamohammadi Elham, Chaleshi Vahid, Rostami Nejad Mohammad, Asadzadeh-Aghdaei Hamid, Reza Zali Mohammad
机构信息
Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterology and Liver Diseases Research Centre, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Prz Gastroenterol. 2018;13(4):293-298. doi: 10.5114/pg.2018.79808. Epub 2018 Dec 11.
INTRODUCTION
Celiac disease (CD) is a chronic inflammatory intestinal disorder. Different immunological factors, including inflammatory cytokines, may play an important role in disease susceptibility.
AIM
To investigate the relationship between -174G/C and -572G/C gene polymorphisms and the serum level of interleukin 6 (IL-6) and susceptibility to CD in the Iranian population.
MATERIAL AND METHODS
In this case-control study blood samples were collected of 105 patients with CD and 106 healthy subjects randomly in 2016 and evaluated by polymerase chain reaction-restriction fragments length polymorphism (PCR-RFLP) method. A sequence was also used to confirm the results of both polymorphisms. The IL-6 concentration was measured using ELISA.
RESULTS
The results showed a significant relationship between polymorphism -572G in CD patients when compared with control subjects by genotype ( = 0.001) and alleles ( = 0.022), respectively. There was no significant relationship between polymorphism 174G and frequency of genotype, but an association of this polymorphism with the frequency of alleles ( = 0.034), age ( = 0.001), and body mass index ( = 0.003) was seen. The serum level of interleukin-6 was significantly associated only with rs1800796 ( < 0.001).
CONCLUSIONS
The results confirm previous studies in different parts of the world and indicate that IL-6 (572G/C) polymorphism may play a role in susceptibility to CD in the Iranian population.
引言
乳糜泻（CD）是一种慢性炎症性肠道疾病。不同的免疫因素，包括炎性细胞因子，可能在疾病易感性中起重要作用。
目的
研究-174G/C和-572G/C基因多态性与伊朗人群血清白细胞介素6（IL-6）水平及乳糜泻易感性之间的关系。
材料与方法
在这项病例对照研究中，于2016年随机收集了105例乳糜泻患者和106例健康受试者的血样，并采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行评估。还使用了一种测序方法来确认两种多态性的结果。采用酶联免疫吸附测定法（ELISA）测量IL-6浓度。
结果
结果显示，与对照组相比，CD患者中-572G多态性在基因型（P = 0.001）和等位基因（P = 0.022）方面均存在显著相关性。174G多态性与基因型频率之间无显著相关性，但该多态性与等位基因频率（P = 0.034）、年龄（P = 0.001）和体重指数（P = 0.003）存在关联。白细胞介素-6的血清水平仅与rs1800796显著相关（P < 0.001）。
结论
结果证实了世界不同地区先前的研究，并表明IL-6（572G/C）多态性可能在伊朗人群乳糜泻易感性中起作用。
Zotero 插件