Preparation and evaluation of a hair wax containing propolis and seed oil for hair growth.

BACKGROUND

Hair growth as a key consumer objective has important role in the hair care products researches. This study was aimed to investigate the effect of a hair wax containing propolis, a resinous mixture produced by honeybees in seed oil base on hair growth.

MATERIALS AND METHODS

The hair wax was designed and formulated compared with marketed brand hair wax and evaluated for pharmaceutical parameters including pH, homogeneity, consistency, spread ability, drug release, and stability. After selection of the best formulation containing 10% ethanolic extract of propolis and 10% seed oil, the hair growth potential was evaluated by application of 1 g hair wax daily on 4 cm area of dorsal side of Wistar rats and compared with controls and standard medication (1 ml of 2% minoxidil). After 30 days treatment, the length and weight of hairs and percentage of hair follicles in different phases of growth in skin biopsies were assessed.

RESULTS

The selected hair wax formulation was stable and easy to wash. The formulation significantly increased hair length on 10, 20, and 30 day compared control group (5.8 ± 0.3 vs. 2.6 ± 0.4, 11.4 ± 0.6 vs. 5.8 ± 0.4, and 17.5 ± 0.5 vs. 12.7 ± 0.4 mm, respectively) and also the weight of newly grown hairs on 30 day (0.056 ± 0.006 vs. 0.043 ± 0.005). It improved hair follicles percentages in anagen phase without any sensitivity reaction.

CONCLUSIONS

The results of this study suggest that the formulated hair wax containing of propolis and seed oil could have significant effect on promoting hair growth.