Shatalebi Mohammad-Ali, Safaeian Leila, Baradaran Azar, Alamdarian Mozhde
Department of Pharmaceutics, Novel Drug Delivery Systems Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan, Iran.
Adv Biomed Res. 2016 Nov 28;5:182. doi: 10.4103/2277-9175.190985. eCollection 2016.
Hair growth as a key consumer objective has important role in the hair care products researches. This study was aimed to investigate the effect of a hair wax containing propolis, a resinous mixture produced by honeybees in seed oil base on hair growth.
The hair wax was designed and formulated compared with marketed brand hair wax and evaluated for pharmaceutical parameters including pH, homogeneity, consistency, spread ability, drug release, and stability. After selection of the best formulation containing 10% ethanolic extract of propolis and 10% seed oil, the hair growth potential was evaluated by application of 1 g hair wax daily on 4 cm area of dorsal side of Wistar rats and compared with controls and standard medication (1 ml of 2% minoxidil). After 30 days treatment, the length and weight of hairs and percentage of hair follicles in different phases of growth in skin biopsies were assessed.
The selected hair wax formulation was stable and easy to wash. The formulation significantly increased hair length on 10, 20, and 30 day compared control group (5.8 ± 0.3 vs. 2.6 ± 0.4, 11.4 ± 0.6 vs. 5.8 ± 0.4, and 17.5 ± 0.5 vs. 12.7 ± 0.4 mm, respectively) and also the weight of newly grown hairs on 30 day (0.056 ± 0.006 vs. 0.043 ± 0.005). It improved hair follicles percentages in anagen phase without any sensitivity reaction.
The results of this study suggest that the formulated hair wax containing of propolis and seed oil could have significant effect on promoting hair growth.
头发生长作为一个关键的消费者目标，在护发产品研究中具有重要作用。本研究旨在调查一种以种子油为基质、含有蜜蜂产生的树脂混合物蜂胶的发蜡对头发生长的影响。
设计并配制该发蜡，并与市售品牌发蜡进行比较，评估其药学参数，包括pH值、均匀性、稠度、铺展性、药物释放和稳定性。在选择了含有10%蜂胶乙醇提取物和10%种子油的最佳配方后，通过每天在Wistar大鼠背部4平方厘米区域涂抹1克发蜡来评估其生发潜力，并与对照组和标准药物（1毫升2%米诺地尔）进行比较。治疗30天后，评估毛发的长度和重量以及皮肤活检中不同生长阶段毛囊的百分比。
所选的发蜡配方稳定且易于清洗。与对照组相比，该配方在第10天、第20天和第30天显著增加了毛发长度（分别为5.8±0.3对2.6±0.4、11.4±0.6对5.8±0.4、17.5±0.5对12.7±0.4毫米），并且在第30天增加了新生长毛发的重量（0.056±0.006对0.043±0.005）。它提高了生长期毛囊的百分比，且无任何过敏反应。
本研究结果表明，所配制的含有蜂胶和种子油的发蜡可能对头发生长有显著促进作用。
