Rodrigues L N, Sousa S F, Silva Rcc, Abreu Mhg, Pires F R, Mesquita R A, Bastos-Rodrigues L, De Marco L, Gomes C C, Gomez R S, Bernardes V F
Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Department of Community and Preventive Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Oral Dis. 2017 May;23(4):471-476. doi: 10.1111/odi.12627. Epub 2017 Feb 8.
The amalgam-associated oral lichenoid lesion (AAOLL) shows clinical and histopathological features similar to oral lichen planus (OLP). Molecular researches to improve knowledge of pathogenesis and clinical behavior of AAOLL are still scarce.
We investigated for the first time the use of loss of heterozygosity (LOH) as a molecular approach for genetic characterization of AAOLL in comparison with OLP and evaluated the cell proliferation index.
The sample comprised nine AAOLLs, 10 OLPs, and eight NOMs matched by patients' gender and age. LOH was assessed using polymorphic microsatellite markers at chromosomes 9p (D9S157, D9S162, D9S171), 11q (D11S1369), and 17p (TP53, AFM238WF2). Cell proliferation was assessed by immunohistochemical expression of Ki-67 (MIB-1). The association between LOH and Ki-67 was investigated.
Loss of heterozygosity occurred in 5/9 AAOLLs and in 2/10 OLPs in at least one marker each, while NOM showed no LOH. Cell proliferation index in AAOLL ranged from 2 to 23%. There was no association between cell proliferation and LOH, independent of the marker.
Our study shows that the profile of molecular changes in AAOLL and OLP, evaluated by LOH and Ki-67 expression, is similar. Additional studies including larger samples should be performed to confirm or to refute our findings.
汞合金相关的口腔苔藓样病变（AAOLL）具有与口腔扁平苔藓（OLP）相似的临床和组织病理学特征。关于提高对AAOLL发病机制和临床行为认识的分子研究仍然很少。
我们首次研究了杂合性缺失（LOH）作为一种分子方法用于AAOLL与OLP的基因特征分析，并评估细胞增殖指数。
样本包括9例AAOLL、10例OLP和8例正常口腔黏膜（NOM），按患者性别和年龄匹配。使用位于9号染色体p臂（D9S157、D9S162、D9S171）、11号染色体q臂（D11S1369）和17号染色体p臂（TP53、AFM238WF2）的多态性微卫星标记评估LOH。通过Ki-67（MIB-1）的免疫组化表达评估细胞增殖。研究LOH与Ki-67之间的关联。
至少在一个标记中，5/9例AAOLL和2/10例OLP出现杂合性缺失，而NOM未显示LOH。AAOLL的细胞增殖指数范围为2%至23%。细胞增殖与LOH之间无关联，与标记无关。
我们的研究表明，通过LOH和Ki-67表达评估的AAOLL和OLP分子变化特征相似。应进行包括更大样本的进一步研究以证实或反驳我们的发现。
