Kazemi Soheila, Baltzer Wendy, Schilke Karl, Mansouri Hadi, Mata John Enrique
Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA; Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA.
School of Chemical, Biological & Environmental Engineering, Oregon State University, Corvallis, OR, USA; School of Chemical, Biological & Environmental Engineering, Oregon State University, Corvallis, OR, USA.
Future Sci OA. 2015 Nov 1;1(4):FSO81. doi: 10.4155/fso.15.81. eCollection 2015 Nov.
Spinal cord regeneration following treatment with a novel membrane-spanning peptide (MSP) expressing the isoleucine-lysine-valine-alanine-valine (IKVAV) epitope was assessed in Balb-c mice. After hemilaminectomy and compression injury, mice were treated with IKVAV, IKVAV-MSP, peptide or vehicle control. Functional improvement was assessed using modified Basso, Beattie, and Bresnahan Scale (mBBB) and spinal cord segments were studied histologically 28 days after injury. IKVAV-MSP group scores increased significantly compared with control groups after 4 weeks of observation (p < 0.05). The number of protoplasmic astrocytes, neurons and muscle bundle size in the IKVAV-MSP mice were significantly increased (p < 0.001; p < 0.05 and p < 0.007; respectively). This study demonstrates that it is possible to promote functional recovery after SCI using bioactive IKVAV presenting cell membrane-spanning peptides.
在Balb-c小鼠中评估了用表达异亮氨酸 - 赖氨酸 - 缬氨酸 - 丙氨酸 - 缬氨酸（IKVAV）表位的新型跨膜肽（MSP）治疗后的脊髓再生情况。在进行半椎板切除术和压迫性损伤后，小鼠分别接受IKVAV、IKVAV - MSP、肽或载体对照治疗。使用改良的Basso、Beattie和Bresnahan评分量表（mBBB）评估功能改善情况，并在损伤后28天对脊髓节段进行组织学研究。观察4周后，IKVAV - MSP组的评分与对照组相比显著增加（p < 0.05）。IKVAV - MSP小鼠中的原浆性星形胶质细胞数量、神经元数量和肌束大小均显著增加（分别为p < 0.001；p < 0.05和p < 0.007）。这项研究表明，使用呈现生物活性IKVAV的跨细胞膜肽有可能促进脊髓损伤后的功能恢复。
