Bazrafkan Sahar, Vatandoost Hassan, Heydari Abbas, Bakhshi Hassan, Panahi-Moghadam Somayeh, Hashemi-Aghdam Saedeh, Mohtarami Fatemeh, Rahimiforoushan Abbas, Anlaandş Sinan, Shayeghi Mansoreh, Oshaghi Mohammad Ali, Abtahi Seyed Mohammad
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Isfahan University of Medical Sciences, Isfahan, Iran.
J Arthropod Borne Dis. 2016 Oct 4;10(4):454-461. eCollection 2016 Dec.
Linear dermatitis is endemic in Iran where most cases occur in the Caspian Sea coast and Fars province. The disease is caused by beetles of the genus which are active from early spring to beginning of autumn although its incidence rises from May to August. The classic taxonomy of spp. is based on the male genitalia that is very complex and needs expertise. In this study, we report a DNA-based method to discriminate and (=syn: , ).
Type specimens were collected from north and south of Iran. Molecular typing of the species was performed using restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR)-amplified fragments of mtDNA-COI.
Sequence analyses of the data obtained in this study showed significant DNA polymorphisms. There were 89 substitutions between COI sequences of the two species. The mtDNA-COI fragment comprises several useful species-specific restriction sites comprising III that could result in distinctively different species-specific PCR-RFLP profiles. The III enzyme cuts the 872 bp PCR amplicon of into 737 and 100 bp and two small nonvisible bands whereas it does not cut amplicon into fragments.
This study demonstrates that molecular typing is useful method and allows one to differentiate between two species and is recommended for discrimination of other species, which morphologically are indistinguishable or very difficult to be distinguished.
线状皮炎在伊朗呈地方性流行，多数病例发生在里海沿岸和法尔斯省。该病由某属甲虫引起，这些甲虫从早春到初秋活动，尽管其发病率从5月到8月有所上升。该属物种的经典分类基于非常复杂且需要专业知识的雄性生殖器。在本研究中，我们报告了一种基于DNA的方法来区分某属的两个物种（=同义词：另两个物种）。
从伊朗北部和南部收集模式标本。使用线粒体DNA-COI的聚合酶链反应（PCR）扩增片段的限制性片段长度多态性（RFLP）分析对物种进行分子分型。
本研究获得的数据的序列分析显示出显著的DNA多态性。两个物种的COI序列之间有89个替换。线粒体DNA-COI片段包含几个有用的物种特异性限制性位点，包括酶III，这可导致明显不同的物种特异性PCR-RFLP图谱。酶III将某物种的872 bp PCR扩增子切割成737 bp和100 bp以及两条小的不可见条带，而它不会将另一物种的扩增子切割成片段。
本研究表明分子分型是一种有用的方法，能够区分两个物种，建议用于鉴别其他在形态上难以区分或非常难以区分的该属物种。
