Hashemi Mohammad, Bahari Gholamreza, Naderi Majid, Sadeghi Bojd Simin, Taheri Mohsen
Cellular and Molecular Research Center, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. Email:
Asian Pac J Cancer Prev. 2016 Nov 1;17(11):4985-4989. doi: 10.22034/APJCP.2016.17.11.4985.
Long non-coding RNAs (lncRNAs) are a novel class of non-protein coding RNAs that are involved in a wide variety of biological processes. There are limited data regarding the impact of lnc-LAMC2-1:1 rs2147578 as well as CASC8 rs10505477 T>C polymorphisms on cancer development. Here we examined for the first time whether rs2147578 and rs10505477 polymorphisms are associated with childhood acute lymphoblastic leukemia (ALL) in a total of 110 cases and 120 healthy controls. Genotyping was achieved by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The rs2147578 variant increased the risk of ALL in codominant (OR=4.33, 95%CI=2.00-9.37, p<0.0001, CG vs CC, and OR=5.81, 95%CI=2.30-14.69, p=0.0002, GG vs CC), dominant (OR=4.63, 95%CI=2.18-9.86, p<0.0001, CG+GG vs CC), overdominant (OR=1.74, 95%CI=1.02-2.97, p=0.0444, CG vs CC+GG) and allele (OR=1.91, 95%CI=1.32-2.77, p=0.0008, G vs C) inheritance models tested. No significant association was found between the CASC8 rs10505477 T>C variant and risk of childhood ALL. In conclusion, the present study revealed that the lnc-LAMC2-1:1 rs2147578 polymorphism may be a risk factor for developing childhood ALL. Further studies with larger sample sizes with different ethnicities are now required to confirm our findings.
长链非编码RNA（lncRNAs）是一类新型的非蛋白质编码RNA，参与多种生物学过程。关于lnc-LAMC2-1:1 rs2147578以及CASC8 rs10505477 T>C多态性对癌症发展的影响，相关数据有限。在此，我们首次在总共110例儿童急性淋巴细胞白血病（ALL）病例和120例健康对照中，研究rs2147578和rs10505477多态性是否与儿童ALL相关。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行基因分型。rs2147578变体在共显性（OR=4.33，95%CI=2.00-9.37，p<0.0001，CG与CC相比，以及OR=5.81，95%CI=2.30-14.69，p=0.0002，GG与CC相比）、显性（OR=4.63，95%CI=2.18-9.86，p<0.0001，CG+GG与CC相比）、超显性（OR=1.74，95%CI=1.02-2.97，p=0.0444，CG与CC+GG相比）和等位基因（OR=1.91，95%CI=1.32-2.77，p=0.0008，G与C相比）遗传模型中增加了ALL风险。未发现CASC8 rs10505477 T>C变体与儿童ALL风险之间存在显著关联。总之，本研究表明lnc-LAMC2-1:1 rs2147578多态性可能是儿童ALL发生的一个风险因素。现在需要进一步开展不同种族、更大样本量的研究来证实我们的发现。
