Ahmed Hanaa H, Aglan Hadeer A, Zaazaa Asmaa M, Shalby Aziza B, El Toumy Sayed A
Hormones Department, National Research Centre, Giza, Egypt. Email:
Asian Pac J Cancer Prev. 2016 Nov 1;17(11):4991-4998. doi: 10.22034/APJCP.2016.17.11.4991.
Objective: This research was conducted to explore mechanisms behind the potency of quercetin in inhibiting colon cancer induced in an experimental model. Materials and Methods: Forty adult male rats of Wistar strain were distributed into 4 groups; a negative control group, a colon cancer bearing group, a quercetin-treated group and a 5-fluorouracil (5-FU)-treated group. Serum TAG72 and GAL3 levels were quantified by ELISA. Colonic Wnt5a and Axin-1 gene expression was estimated by PCR. In addition, colonic tissues were subjected to immunohistochemical examination of Bax expression and histological investigation of histopathological alterations. Results: Quercetin elicited significant reduction in serum TAG72 and GAL3 levels, in addition to significant suppression of colonic Wnt5a gene expression and amplification of colonic Axin-1 gene expression. Also, it caused moderate positive reaction for Bax in mucosal epithelium. Conclusion: The present research provides experimental evidence about the activity of quercetin in the colon cancer of rats. Inhibitory effects on cancer development might be ascribable to regulatory action on Wnt signaling and induction of apoptosis.
本研究旨在探究槲皮素在实验模型中抑制结肠癌的潜在机制。材料与方法：将40只成年雄性Wistar大鼠分为4组；阴性对照组、荷结肠癌组、槲皮素治疗组和5-氟尿嘧啶（5-FU）治疗组。通过酶联免疫吸附测定法（ELISA）定量血清TAG72和GAL3水平。通过聚合酶链反应（PCR）评估结肠Wnt5a和Axin-1基因表达。此外，对结肠组织进行Bax表达的免疫组织化学检查和组织病理学改变的组织学研究。结果：槲皮素除了能显著抑制结肠Wnt5a基因表达和扩增结肠Axin-1基因表达外，还能显著降低血清TAG72和GAL3水平。此外，它还导致黏膜上皮中Bax呈中度阳性反应。结论：本研究为槲皮素在大鼠结肠癌中的活性提供了实验证据。对癌症发展的抑制作用可能归因于对Wnt信号通路的调节作用和细胞凋亡的诱导。
