Oikawa S, Inuzuka C, Kuroki M, Matsuoka Y, Kosaki G, Nakazato H
Suntory Institute for Biomedical Research, Osaka, Japan.
Biochem Biophys Res Commun. 1989 Oct 16;164(1):39-45. doi: 10.1016/0006-291x(89)91679-3.
Cell adhesion activity of carcinoembryonic antigen (CEA) and non-specific cross-reacting antigen (NCA) has been analysed by using CHO cells which had been transfected with cDNAs and are ectopically expressing each antigen on their surface. CEA expressing CHO tended to aggregate easily within 30 min after being suspended by trypsinization. Cell adhesion assay between 51Cr labelled cells and monolayered cells showed both homophilic and heterophilic interaction, the extent of which was CEA-CEA much greater than CEA-NCA greater than NCA-NCA. These reactions were completely inhibited by Fab' fragment of anti-CEA antibody. The results strongly suggested that CEA and NCA function as Ca++ independent cell adhesion molecules by homophilic and heterophilic interactions.
通过使用已用cDNA转染并在其表面异位表达每种抗原的CHO细胞，分析了癌胚抗原（CEA）和非特异性交叉反应抗原（NCA）的细胞粘附活性。表达CEA的CHO细胞在胰蛋白酶消化悬浮后30分钟内易于聚集。51Cr标记细胞与单层细胞之间的细胞粘附试验显示了同种亲和和异种亲和相互作用，其程度为CEA-CEA远大于CEA-NCA大于NCA-NCA。这些反应被抗CEA抗体的Fab'片段完全抑制。结果强烈表明，CEA和NCA通过同种亲和和异种亲和相互作用作为Ca++非依赖性细胞粘附分子发挥作用。
